真菌病原体光滑念珠菌对氧化应激的高抗性由单一过氧化氢酶Cta1p介导，并受转录因子Yap1p、Skn7p、Msn2p和Msn4p控制。
High resistance to oxidative stress in the fungal pathogen Candida glabrata is mediated by a single catalase, Cta1p, and is controlled by the transcription factors Yap1p, Skn7p, Msn2p, and Msn4p.
作者信息
Cuéllar-Cruz Mayra, Briones-Martin-del-Campo Marcela, Cañas-Villamar Israel, Montalvo-Arredondo Javier, Riego-Ruiz Lina, Castaño Irene, De Las Peñas Alejandro
机构信息
División de Biología Molecular, Instituto Potosino de Investigación Científica y Tecnológica, San Luis Potosí, México.
出版信息
Eukaryot Cell. 2008 May;7(5):814-25. doi: 10.1128/EC.00011-08. Epub 2008 Mar 28.
Abstract
We characterized the oxidative stress response of Candida glabrata to better understand the virulence of this fungal pathogen. C. glabrata could withstand higher concentrations of H(2)O(2) than Saccharomyces cerevisiae and even Candida albicans. Stationary-phase cells were extremely resistant to oxidative stress, and this resistance was dependent on the concerted roles of stress-related transcription factors Yap1p, Skn7p, and Msn4p. We showed that growing cells of C. glabrata were able to adapt to high levels of H(2)O(2) and that this adaptive response was dependent on Yap1p and Skn7p and partially on the general stress transcription factors Msn2p and Msn4p. C. glabrata has a single catalase gene, CTA1, which was absolutely required for resistance to H(2)O(2) in vitro. However, in a mouse model of systemic infection, a strain lacking CTA1 showed no effect on virulence.
摘要
我们对光滑念珠菌的氧化应激反应进行了表征，以更好地了解这种真菌病原体的毒力。与酿酒酵母甚至白色念珠菌相比，光滑念珠菌能够耐受更高浓度的过氧化氢。稳定期细胞对氧化应激具有极强的抗性，这种抗性依赖于应激相关转录因子Yap1p、Skn7p和Msn4p的协同作用。我们发现，光滑念珠菌的生长细胞能够适应高水平的过氧化氢，这种适应性反应依赖于Yap1p和Skn7p，部分依赖于一般应激转录因子Msn2p和Msn4p。光滑念珠菌有一个单一的过氧化氢酶基因CTA1，该基因是体外抵抗过氧化氢绝对必需的。然而，在系统性感染的小鼠模型中，缺乏CTA1的菌株对毒力没有影响。
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