Lançon A, Delmas D, Osman H, Thénot J-P, Jannin B, Latruffe N
Laboratoire de Biologie Moléculaire et Cellulaire, Université de Bourgogne, 6 boulevard Gabriel, 21000 Dijon, France.
Biochem Biophys Res Commun. 2004 Apr 16;316(4):1132-7. doi: 10.1016/j.bbrc.2004.02.164.
This work reports significant advances on the transport in hepatic cells of resveratrol, a natural polyphenol with potent protective properties. First, we describe a new simple technique to qualitatively follow resveratrol cell uptake and intracellular distribution, based on resveratrol fluorescent properties. Second, the time-course study and the quantification of (3)H-labelled resveratrol uptake have been performed using human hepatic derived cells (HepG2 tumor cells) and hepatocytes. The temperature-dependence of the kinetics of uptake as well as the cis-inhibition experiments agree with the involvement of a carrier-mediated transport in addition to passive diffusion. The decrease of passive uptake resulting from resveratrol binding to serum proteins brings to light a mediated mechanism in physiological situation.
