Prat-Larquemin Lydie, Oppert Jean-Michel, Clément Karine, Hainault Isabelle, Basdevant Arnaud, Guy-Grand Bernard, Quignard-Boulangé Annie
Université Paris VI, Hôtel-Dieu, Parvis Notre-Dame, Paris, France.
Obes Res. 2004 Mar;12(3):556-61. doi: 10.1038/oby.2004.63.
To investigate AGT secretion in cultured adipocytes from obese patients and its relationship with obesity-related phenotypes, blood pressure, and the M235T polymorphism in the AGT gene.
Measurements, including anthropometry, body composition (DXA), and blood pressure, were performed in 61 overweight or obese women (BMI: 28 to 68 kg/m(2)). A subcutaneous abdominal adipose tissue biopsy was used for adipocyte size determination and quantification of AGT secretion in the medium of cultured adipocytes. AGT M235T genotype was determined using polymerase chain reaction-restriction fragment length polymorphism.
Adipose secretion of the AGT protein (range, 140 to 2575 ng/10(6) cells/24 h) was not significantly correlated with BMI, body fat, or blood pressure and did not vary according to the M235T polymorphism in the AGT gene. However, the AGT M235T polymorphism was associated with adipocyte size (111.6 +/- 2.8, 108.8 +/- 1.9, 118.2 +/- 2.6 micro m in MM, MT, and TT genotypes, respectively; p < 0.01) after adjustment for age and fat mass. An association between the AGT M235T polymorphism and adipocyte size (p < 0.02 adjusted for sex, age, and BMI) was found in another independent sample of 106 obese subjects (sex ratio, M/F 16/90; BMI, 29 to 70 kg/m(2)).
In cultured adipocytes from obese subjects, AGT secretion was not associated with body fat phenotypes, blood pressure, or fat cell size. However, results from two independent studies suggest an association between the AGT M235T polymorphism and adipocyte size.
研究肥胖患者培养的脂肪细胞中血管紧张素原（AGT）的分泌情况及其与肥胖相关表型、血压和AGT基因M235T多态性的关系。
对61名超重或肥胖女性（体重指数：28至68kg/m²）进行测量，包括人体测量、身体成分分析（双能X线吸收法）和血压测量。采用皮下腹部脂肪组织活检来确定脂肪细胞大小，并对培养的脂肪细胞培养基中的AGT分泌进行定量分析。使用聚合酶链反应-限制性片段长度多态性方法确定AGT M235T基因型。
AGT蛋白的脂肪分泌量（范围为140至2575ng/10⁶细胞/24小时）与体重指数、体脂或血压无显著相关性，且不随AGT基因的M235T多态性而变化。然而，在调整年龄和脂肪量后，AGT M235T多态性与脂肪细胞大小相关（MM、MT和TT基因型的脂肪细胞大小分别为111.6±2.8、108.8±1.9、118.2±2.6μm；p<0.01）。在另一个由106名肥胖受试者组成的独立样本中（性别比为男性/女性16/90；体重指数为29至70kg/m²），发现AGT M235T多态性与脂肪细胞大小相关（经性别、年龄和体重指数调整后p<0.02）。
在肥胖受试者培养的脂肪细胞中，AGT分泌与体脂表型、血压或脂肪细胞大小无关。然而，两项独立研究的结果表明AGT M235T多态性与脂肪细胞大小之间存在关联。
