Darain Farzana, Park Deog-Su, Park Jang-Su, Shim Yoon-Bo
Department of Chemistry and Center for Innovative Bio-Physio Sensor Technology, Pusan National University, Busan 609-735, South Korea.
Biosens Bioelectron. 2004 May 15;19(10):1245-52. doi: 10.1016/j.bios.2003.11.014.
A simple and direct immunosensor for the determination of carp (Carassius auratus) vitellogenin (Vtg), a female-specific protein, has been proposed based on an antibody-captured conducting polymer-coated electrode. The monoclonal antibody specific to carp (C. auratus) Vtg was immobilized by covalent coupling to the carboxylic acid group on the polymer. The antibody immobilization and antibody-antigen interaction have been demonstrated by means of quartz crystal microbalance and impedance spectroscopic techniques. The impedance change occurred at the sensor surface due to the specific immuno-interaction was utilized to determine Vtg. The sensor showed high selectivity and sensitive response to Vtg in a buffered medium without redox probe. Vtg was determined in the linear range from 1.0 to 8.0 microg/l with the standard deviation of +/-0.13 (n =3) and the detection limit was determined to be 0.42 microg/l. This method was applied to the determination of Vtg in real male and female carp (C. auratus) serum samples.
基于抗体捕获的导电聚合物涂层电极，提出了一种简单直接的免疫传感器，用于测定鲤鱼（Carassius auratus）卵黄蛋白原（Vtg），这是一种雌性特异性蛋白质。通过与聚合物上的羧酸基团共价偶联，固定了对鲤鱼（C. auratus）Vtg具有特异性的单克隆抗体。利用石英晶体微天平及阻抗光谱技术证明了抗体的固定以及抗体 - 抗原相互作用。利用传感器表面因特异性免疫相互作用而发生的阻抗变化来测定Vtg。该传感器在无氧化还原探针的缓冲介质中对Vtg表现出高选择性和灵敏响应。Vtg的测定线性范围为1.0至8.0微克/升，标准偏差为±0.13（n = 3），检测限为0.42微克/升。该方法应用于实际雄性和雌性鲤鱼（C. auratus）血清样品中Vtg的测定。
