Vallet Sophie, Gagneur Arnaud, Talbot Pierre J, Legrand Marie-Christine, Sizun Jacques, Picard Bertrand
Laboratoire de Virologie, Department of Microbiology, Centre hospitalier Universitaire Morvan, 5 avenue Foch, 29200 Brest Cedex, France.
Mol Cell Probes. 2004 Apr;18(2):75-80. doi: 10.1016/j.mcp.2003.09.005.
A novel human Coronavirus (HCoV) was this year recognized as the etiological agent of the Severe Acute Respiratory Syndrome. Two other HCoV (HCoV-229E and HCoV-OC43) have been known for 30 years. HCoV-229E has been recently involved in nosocomial respiratory viral infections in high-risk children. However, their diagnosis is not routinely performed. Currently, reliable immunofluorescence and cell culture methodologies are not available. As part of a four-year epidemiological study in a Pediatric and Neonatal Intensive care unit, we have performed and demonstrated the reliability of a reverse transcription-PCR-hybridization assay to detect HCoV of the 229E antigenic group in 2028 clinical respiratory specimens. In hospitalized children (children and newborns) and staff members we found a high incidence of HcoV-229E infection. This reverse transcription-PCR-hybridization assay gave a high specificity and a sensitivity of 0.5 50% Tissue Culture Infective Dose per ml. This technique is reliable and its application for screening large number of clinical samples would improve the diagnosis of HCoVs respiratory infection and our knowledge of these viruses epidemiology.
一种新型人类冠状病毒（HCoV）今年被确认为严重急性呼吸综合征的病原体。另外两种HCoV（HCoV - 229E和HCoV - OC43）已被知晓30年。HCoV - 229E最近与高危儿童的医院内呼吸道病毒感染有关。然而，它们的诊断并非常规进行。目前，尚无可靠的免疫荧光和细胞培养方法。作为在儿科和新生儿重症监护病房进行的为期四年的流行病学研究的一部分，我们进行并证明了逆转录 - PCR杂交检测法在2028份临床呼吸道标本中检测229E抗原组HCoV的可靠性。在住院儿童（儿童和新生儿）及工作人员中，我们发现HCoV - 229E感染的发生率很高。这种逆转录 - PCR杂交检测法具有高特异性，灵敏度为每毫升0.5 50%组织培养感染剂量。该技术可靠，其在大量临床样本筛查中的应用将改善HCoV呼吸道感染的诊断以及我们对这些病毒流行病学的了解。
