Vandecasteele Stefaan Johan, Peetermans Willy Eduard, Carbonez An, Van Eldere Johan
Infectious Diseases Research Group, Department of Microbiology and Immunology, Rega Institute for Medical Research and Internal Medicine and Infectious Diseases, University Hospital Leuven, Leuven, Belgium.
J Bacteriol. 2004 Apr;186(8):2236-9. doi: 10.1128/JB.186.8.2236-2239.2004.
Foreign-body infection (FBI) is notoriously resistant to eradication by antibiotic treatment. It is hypothesized that reduced bacterial metabolic activity contributes to this resistance. We examined the metabolic activity of Staphylococcus epidermidis in 204 samples recovered during in vitro foreign-body colonization and in 424 samples recovered during in vivo FBI in a rat model. Metabolic activity was measured by determining the amount of 16S rRNA per genome by quantitative PCR. The initial foreign-body-associated growth proved to be a metabolically active process, both in vitro and in vivo. The initial 16S rRNA content was similar to that observed during in vitro exponential-growth phase. However, during late in vivo FBI, a 114-fold (P << 0.0001) decrease in the 16S rRNA content was observed, indicating that there was markedly decreased metabolic activity. This decreased metabolic activity during late FBI can explain at least in part why such infections are so difficult to eradicate with conventional antibiotic treatment.
异物感染（FBI）对抗生素治疗的根除具有 notoriously 的抗性。据推测，细菌代谢活性降低是导致这种抗性的原因。我们检测了在大鼠模型中体外异物定植期间回收的204个样本以及体内FBI期间回收的424个样本中表皮葡萄球菌的代谢活性。通过定量PCR测定每个基因组中16S rRNA的量来测量代谢活性。最初的异物相关生长在体外和体内均被证明是一个代谢活跃的过程。最初的16S rRNA含量与体外指数生长期观察到的相似。然而，在体内FBI后期，观察到16S rRNA含量下降了114倍（P << 0.0001），表明代谢活性明显降低。FBI后期这种代谢活性降低至少可以部分解释为什么此类感染难以用传统抗生素治疗根除。
