Warwick S, Wilks M, Hennessy E, Powell-Tuck J, Small M, Sharp J, Millar M R
Department of Microbiology, Barts and The London NHS Trust, London, UK.
J Clin Microbiol. 2004 Apr;42(4):1402-8. doi: 10.1128/JCM.42.4.1402-1408.2004.
Many central vascular catheters (CVCs) are removed unnecessarily because current diagnostic methods for CVC-associated infection are unreliable. A quantitative PCR assay using primers and probe targeted to bacterial 16S ribosomal DNA was used to measure the levels of bacterial DNA in blood samples drawn through the CVC in a population of patients receiving intravenous nutrition. Bacterial DNA concentrations were raised in 16 of 16 blood samples taken during episodes of probable bacterial CVC-associated infection. Bacterial DNA concentrations were raised in 4 of 29 episodes in which bacterial CVC-associated infection was unlikely. The use of this technique has the potential to substantially reduce the unnecessary removal of CVCs.
许多中心静脉导管（CVC）被不必要地拔除，因为目前用于诊断CVC相关感染的方法并不可靠。在接受静脉营养的患者群体中，使用针对细菌16S核糖体DNA的引物和探针进行定量PCR检测，以测量通过CVC采集的血样中细菌DNA的水平。在可能发生细菌CVC相关感染的发作期间采集的16份血样中，有16份的细菌DNA浓度升高。在不太可能发生细菌CVC相关感染的29次发作中，有4次细菌DNA浓度升高。使用这种技术有可能大幅减少CVC的不必要拔除。
