两种广泛用于检测羊水、血液和组织中弓形虫的聚合酶链反应（PCR）引物系统的比较。
Comparison of two widely used PCR primer systems for detection of toxoplasma in amniotic fluid, blood, and tissues.
作者信息
Chabbert Elisabeth, Lachaud Laurence, Crobu Lucien, Bastien Patrick
机构信息
Laboratoire de Parasitologie-Mycologie, Centre Hospitalier Universitaire, Montpellier, France.
出版信息
J Clin Microbiol. 2004 Apr;42(4):1719-22. doi: 10.1128/JCM.42.4.1719-1722.2004.
Abstract
The PCR diagnosis of toxoplasmosis suffers from lack of standardization. Interlaboratory comparative studies of PCR methods have been performed, but intralaboratory comparisons are scarce. Here, we optimized and compared the technical performances of two PCR primer systems widely used for Toxoplasma detection. The differences between the two methods were visible only at low parasite concentrations (< or = 1 Toxoplasma genome per reaction tube). Nevertheless, when clinical samples were tested, both methods significantly differed in their technical sensitivities and specificities. Only one method appeared adequate for samples containing blood or tissue.
摘要
弓形虫病的聚合酶链反应（PCR）诊断缺乏标准化。已经开展了PCR方法的实验室间比较研究，但实验室内部的比较却很少。在此，我们优化并比较了两种广泛用于检测弓形虫的PCR引物系统的技术性能。两种方法之间的差异仅在低寄生虫浓度时（每个反应管中≤1个弓形虫基因组）才可见。然而，在检测临床样本时，两种方法在技术敏感性和特异性方面存在显著差异。只有一种方法似乎适用于含有血液或组织的样本。
相似文献
1
Comparison of two widely used PCR primer systems for detection of toxoplasma in amniotic fluid, blood, and tissues.两种广泛用于检测羊水、血液和组织中弓形虫的聚合酶链反应（PCR）引物系统的比较。
J Clin Microbiol. 2004 Apr;42(4):1719-22. doi: 10.1128/JCM.42.4.1719-1722.2004.
2
[Detection of congenital toxoplasmosis in utero with a polymerase chain reaction on amniotic fluid].[通过羊水聚合酶链反应检测子宫内先天性弓形虫病]
Wiad Parazytol. 2002;48(3):311-5.
3
Comparison between two amplification sets for molecular diagnosis of toxoplasmosis by real-time PCR.通过实时PCR对弓形虫病进行分子诊断时两组扩增体系的比较。
J Clin Microbiol. 2006 Mar;44(3):720-4. doi: 10.1128/JCM.44.3.720-724.2006.
4
Follow-up of infants with congenital toxoplasmosis detected by polymerase chain reaction analysis of amniotic fluid.通过羊水聚合酶链反应分析检测出的先天性弓形虫病婴儿的随访
Eur J Clin Microbiol Infect Dis. 1998 Dec;17(12):853-8. doi: 10.1007/s100960050206.
5
A new set of primers for the detection of Toxoplasma gondii in amniotic fluid using polymerase chain reaction.一组用于通过聚合酶链反应检测羊水中弓形虫的新型引物。
FEMS Microbiol Lett. 1996 Apr 15;138(1):11-5. doi: 10.1111/j.1574-6968.1996.tb08127.x.
6
Evaluation of polymerase chain reaction on amniotic fluid for diagnosis of congenital toxoplasmosis.羊水聚合酶链反应用于先天性弓形虫病诊断的评估
J Egypt Soc Parasitol. 2009 Aug;39(2):541-50.
7
Prenatal diagnosis of congenital toxoplasmosis: a multicenter evaluation of different diagnostic parameters.先天性弓形虫病的产前诊断：不同诊断参数的多中心评估
Am J Obstet Gynecol. 1999 Oct;181(4):843-7. doi: 10.1016/s0002-9378(99)70311-x.
8
[Real-time quantitative PCR for toxoplasmosis diagnosis].[用于弓形虫病诊断的实时定量聚合酶链反应]
Ann Biol Clin (Paris). 2005 Jan-Feb;63(1):67-73.
9
[Prenatal diagnostics of Toxoplasma gondii invasion by quantitative method of PCR-TaqMann].[采用PCR-TaqMann定量法进行弓形虫感染的产前诊断]
Wiad Parazytol. 2001;47 Suppl 1:91-7.
10
Polymerase chain reaction in the diagnosis of congenital toxoplasmosis: more than two decades of development and evaluation.聚合酶链反应在先天性弓形虫病诊断中的应用：二十多年的发展和评估。
Parasitol Res. 2011 Mar;108(3):505-12. doi: 10.1007/s00436-010-2245-8. Epub 2011 Jan 11.
引用本文的文献
1
Systematic Review and Meta-Analysis of Congenital Toxoplasmosis Diagnosis: Advances and Challenges.先天性弓形虫病诊断的系统评价与Meta分析：进展与挑战
J Trop Med. 2024 Feb 21;2024:1514178. doi: 10.1155/2024/1514178. eCollection 2024.
2
Comparison of real-time PCR and nested PCR for toxoplasmosis diagnosis in toxoplasmic retinochoroiditis patients.实时 PCR 和巢式 PCR 在弓形虫视网膜炎患者中的诊断比较。
BMC Infect Dis. 2021 Nov 23;21(1):1180. doi: 10.1186/s12879-021-06873-3.
3
Inhibition of polymerase chain reaction: Pathogen-specific controls are better than human gene amplification.抑制聚合酶链反应：病原体特异性对照优于人类基因扩增。
PLoS One. 2019 Sep 27;14(9):e0219276. doi: 10.1371/journal.pone.0219276. eCollection 2019.
4
Enhancing the detection of an anti-SAG1 scFv-alkaline phosphatase immunoconjugate.增强抗SAG1单链抗体片段-碱性磷酸酶免疫偶联物的检测
Biotechnol Rep (Amst). 2019 Jul 5;23:e00360. doi: 10.1016/j.btre.2019.e00360. eCollection 2019 Sep.
5
Seromolecular assess of infection in pregnant women and neonatal umbilical cord blood.孕妇及新生儿脐带血感染的血清分子评估
EXCLI J. 2019 Jan 2;18:1-7. eCollection 2019.
6
Performance of 2 Polymerization Protocols Targeting Cloned Toxoplasma Parasites.针对克隆的弓形虫寄生虫的两种聚合酶链反应方案的性能
Open Access Maced J Med Sci. 2018 Sep 22;6(9):1577-1580. doi: 10.3889/oamjms.2018.400. eCollection 2018 Sep 25.
7
Toxoplasma gondii Infection and Chronic Liver Diseases: Evidence of an Association.弓形虫感染与慢性肝病：关联证据
Trop Med Infect Dis. 2016 Nov 1;1(1):7. doi: 10.3390/tropicalmed1010007.
8
Molecular diagnosis of symptomatic toxoplasmosis: a 9-year retrospective and prospective study in a referral laboratory in São Paulo, Brazil.有症状弓形虫病的分子诊断：在巴西圣保罗一家转诊实验室进行的为期9年的回顾性和前瞻性研究。
Braz J Infect Dis. 2017 Nov-Dec;21(6):638-647. doi: 10.1016/j.bjid.2017.07.003. Epub 2017 Sep 29.
9
Pathology, clinical signs, and tissue distribution of in experimentally infected reindeer ().实验感染驯鹿（）中的病理学、临床症状及组织分布 。 （注：原文括号内内容不完整，可能影响准确理解）
Int J Parasitol Parasites Wildl. 2017 Aug 15;6(3):234-240. doi: 10.1016/j.ijppaw.2017.08.004. eCollection 2017 Dec.
10
Prenatal education for congenital toxoplasmosis.先天性弓形虫病的产前教育。
Cochrane Database Syst Rev. 2015 Oct 23;2015(10):CD006171. doi: 10.1002/14651858.CD006171.pub4.
本文引用的文献
1
Molecular diagnostics in clinical parasitology and mycology: limits of the current polymerase chain reaction (PCR) assays and interest of the real-time PCR assays.临床寄生虫学和真菌学中的分子诊断：当前聚合酶链反应（PCR）检测的局限性及实时PCR检测的优势
Clin Microbiol Infect. 2003 Jun;9(6):505-11. doi: 10.1046/j.1469-0691.2003.00677.x.
2
Comparison of real-time PCR detection methods for B1 and P30 genes of Toxoplasma gondii.弓形虫B1和P30基因实时荧光定量PCR检测方法的比较
Diagn Microbiol Infect Dis. 2003 Apr;45(4):269-71. doi: 10.1016/s0732-8893(02)00549-7.
3
Comparison of two DNA targets for the diagnosis of Toxoplasmosis by real-time PCR using fluorescence resonance energy transfer hybridization probes.使用荧光共振能量转移杂交探针通过实时PCR诊断弓形虫病时两种DNA靶标的比较
BMC Infect Dis. 2003 May 2;3:7. doi: 10.1186/1471-2334-3-7.
4
Optimization and evaluation of a PCR assay for detecting toxoplasmic encephalitis in patients with AIDS.用于检测艾滋病患者弓形虫性脑炎的聚合酶链反应检测方法的优化与评估
J Clin Microbiol. 2002 Dec;40(12):4499-503. doi: 10.1128/JCM.40.12.4499-4503.2002.
5
Comparison of an industry-derived LCx Chlamydia pneumoniae PCR research kit to in-house assays performed in five laboratories.一种源自行业的LCx肺炎衣原体PCR研究试剂盒与五个实验室进行的内部检测方法的比较。
J Clin Microbiol. 2002 Jul;40(7):2357-62. doi: 10.1128/JCM.40.7.2357-2362.2002.
6
Molecular diagnosis of toxoplasmosis.弓形虫病的分子诊断
Trans R Soc Trop Med Hyg. 2002 Apr;96 Suppl 1:S205-15. doi: 10.1016/s0035-9203(02)90078-7.
7
Comparison of six PCR methods using peripheral blood for detection of canine visceral leishmaniasis.六种利用外周血检测犬内脏利什曼病的聚合酶链反应方法的比较
J Clin Microbiol. 2002 Jan;40(1):210-5. doi: 10.1128/JCM.40.1.210-215.2002.
8
Quality control for the diagnosis of Toxoplasma gondii reactivation in SCT patients using PCR assays.使用聚合酶链反应（PCR）检测法对造血干细胞移植（SCT）患者弓形虫再激活诊断的质量控制
Bone Marrow Transplant. 2001 Sep;28(5):527-8. doi: 10.1038/sj.bmt.1703175.
9
Development of a real-time PCR assay for detection of Toxoplasma gondii in pig and mouse tissues.用于检测猪和小鼠组织中弓形虫的实时荧光定量PCR检测方法的建立。
J Clin Microbiol. 2001 Jun;39(6):2065-71. doi: 10.1128/JCM.39.6.2065-2071.2001.
10
Comparison of various sample preparation methods for PCR diagnosis of visceral leishmaniasis using peripheral blood.使用外周血进行内脏利什曼病PCR诊断的各种样本制备方法的比较
J Clin Microbiol. 2001 Feb;39(2):613-7. doi: 10.1128/JCM.39.2.613-617.2001.
Zotero 插件