孕妇及新生儿脐带血感染的血清分子评估

Seromolecular assess of infection in pregnant women and neonatal umbilical cord blood.

作者信息

Rashno Mohammad Menati, Fallahi Shirzad, Arab-Mazar Zahra, Dana Hassan

机构信息

Department of Biotechnology, Damghan Branch, Islamic Azad University, Damghan, Iran.

Department of Medical Parasitology and Mycology, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran.

出版信息

EXCLI J. 2019 Jan 2;18:1-7. eCollection 2019.

Toxoplasmosis is considered as one of the most prevalent human parasitic infections that can be transmitted from mother to the fetus. The onset of toxoplasmosis during pregnancy has clinical complications including spontaneous abortion, preterm labor, stillbirth and fetal abnormalities. The aim of this study was to investigate the prevalence of Toxoplasmosis infection in pregnant women and their infants in Lorestan province, Western Iran. Blood and sera samples were collected from 98 pregnant women and their infants. All collected samples were examined for infection by serological tests (ELISA IgM & IgG) and PCR assay. Among the 98 samples of mother and umbilical cord prevalence of anti- IgG, was 34/98 (34.69 %) and 33/98 (33.67 %), respectively. All pregnant women were negative for, anti- IgM while it was found in 5/98 (5.1 %) of umbilical cords. Based on PCR analysis, infection was detected in 5 (5.1 %) and 7 (7.14 %) of mother and umbilical cords, respectively. Molecular test along with evaluation of IgM (P <0.001) and IgG (P = 0.001) were significantly correlated.

弓形虫病被认为是最常见的可由母亲传播给胎儿的人类寄生虫感染之一。孕期弓形虫病的发作会引发包括自然流产、早产、死产和胎儿畸形在内的临床并发症。本研究的目的是调查伊朗西部洛雷斯坦省孕妇及其婴儿中弓形虫感染的患病率。从98名孕妇及其婴儿中采集了血液和血清样本。所有采集的样本均通过血清学检测（ELISA IgM和IgG）和PCR检测进行感染检测。在98份母亲和脐带样本中，抗IgG的患病率分别为34/98（34.69%）和33/98（33.67%）。所有孕妇的抗IgM均为阴性，而在5/98（5.1%）的脐带中检测到抗IgM。基于PCR分析，分别在5名（5.1%）母亲和7名（7.14%）脐带中检测到感染。分子检测与IgM（P<0.001）和IgG（P = 0.001）的评估显著相关。