植物中的转基因整合：在杂乱的基因组中戳洞还是补洞？
Transgene integration in plants: poking or patching holes in promiscuous genomes?
作者信息
Somers David A, Makarevitch Irina
机构信息
Department of Agronomy and Plant Genetics, Plant Molecular Genetics Institute, University of Minnesota, 1991 Upper Buford Circle, St. Paul, Minnesota, USA.
出版信息
Curr Opin Biotechnol. 2004 Apr;15(2):126-31. doi: 10.1016/j.copbio.2004.02.007.
Transgene integration in plants transformed by either Agrobacterium or direct DNA delivery methods occurs through illegitimate recombination (IR). The precise mechanism(s) for IR-mediated transgene integration and the role of host double-strand break repair enzymes remain unknown. A recent wealth of sequenced transgene loci and investigations aimed at genetically dissecting transgene integration mechanism(s) have provided new insights into the process.
通过农杆菌或直接DNA导入方法转化的植物中，转基因整合是通过非同源重组（IR）发生的。IR介导的转基因整合的确切机制以及宿主双链断裂修复酶的作用仍然未知。最近大量已测序的转基因位点以及旨在对转基因整合机制进行遗传剖析的研究为这一过程提供了新的见解。
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