Institute of Bioengineering, Zhejiang Sci-Tech University, Road 2, 310018, Xiasha Hangzhou, PR China.
Int J Syst Evol Microbiol. 2010 Jan;60(Pt 1):8-14. doi: 10.1099/ijs.0.008532-0. Epub 2009 Jul 30.
Bacillus mucilaginosus and Bacillus edaphicus were reclassified based on their 16S rRNA and gyrB gene sequences, DNA-DNA hybridization, fatty acid methyl esters and other taxonomic characteristics. Phylogenetic analysis based on 16S rRNA and gyrB gene sequences indicated that strains of B. mucilaginosus and B. edaphicus were members of the genus Paenibacillus, with over 90.4 % and 70.3 % sequence similarity, respectively. Their DNA G+C contents were 54.5-56.8 mol%. The DNA-DNA relatedness values of B. edaphicus VKPM B-7517(T) with B. mucilaginosus KNP414 and B. mucilaginosus CGMCC 1.236 were 89.2 % and 88.7 %, respectively. The major isoprenoid quinone of B. mucilaginosus and B. edaphicus was MK-7 (94.1-95.7 %). The peptidoglycan type was A1gamma (meso-diaminopimelic acid) and the major polar lipids were phosphatidylglycerol and diphosphatidylglycerol. The major fatty acids were anteiso-C(15 : 0), C(16 : 1)omega11c and C(16 : 0). Phenotypic features and fatty acid profiles supported the similarity of B. mucilaginosus and B. edaphicus to Paenibacillus validus CCTCC 95016(T) and confirmed their relationship with members of the genus Paenibacillus. Therefore, it is proposed that Bacillus mucilaginosus and Bacillus edaphicus be transferred to the genus Paenibacillus as Paenibacillus mucilaginosus comb. nov. (type strain HSCC 1605(T)=VKPM B-7519(T)=VKM B-1480D(T)=CIP 105815(T)=KCTC 3870(T)) and Paenibacillus edaphicus comb. nov. (type strain VKPM B-7517(T)=DSM 12974(T)=CIP 105814(T)), respectively.
基于 16S rRNA 和 gyrB 基因序列、DNA-DNA 杂交、脂肪酸甲酯和其他分类特征，对粘质沙雷氏菌和土壤沙雷氏菌进行了重新分类。基于 16S rRNA 和 gyrB 基因序列的系统发育分析表明，粘质沙雷氏菌和土壤沙雷氏菌菌株均为类芽孢杆菌属的成员，序列相似性分别超过 90.4%和 70.3%。它们的 DNA G+C 含量为 54.5-56.8mol%。土壤沙雷氏菌 VKPM B-7517(T)与粘质沙雷氏菌 KNP414 和粘质沙雷氏菌 CGMCC 1.236 的 DNA-DNA 相关值分别为 89.2%和 88.7%。粘质沙雷氏菌和土壤沙雷氏菌的主要类异戊二烯醌为 MK-7（94.1-95.7%）。肽聚糖类型为 A1gamma（meso-二氨基庚二酸），主要极性脂为磷脂酰甘油和双磷脂酰甘油。主要脂肪酸为anteiso-C(15:0)、C(16:1)omega11c 和 C(16:0)。表型特征和脂肪酸图谱支持粘质沙雷氏菌和土壤沙雷氏菌与类芽孢杆菌有效种（Paenibacillus validus） CCTCC 95016(T)的相似性，并证实了它们与类芽孢杆菌属成员的关系。因此，建议将粘质沙雷氏菌和土壤沙雷氏菌转移到类芽孢杆菌属中，分别作为粘质沙雷氏菌（Paenibacillus mucilaginosus）组合新种（模式菌株 HSCC 1605(T)=VKPM B-7519(T)=VKM B-1480D(T)=CIP 105815(T)=KCTC 3870(T)）和土壤沙雷氏菌（Paenibacillus edaphicus）组合新种（模式菌株 VKPM B-7517(T)=DSM 12974(T)=CIP 105814(T)）。
