Lauzon Hilary A M, Garcia-Maruniak Alejandra, de A Zanotto Paolo M, Clemente José C, Herniou Elisabeth A, Lucarotti Christopher J, Arif Basil M, Maruniak James E
Canadian Forest Service, Great Lakes Forestry Centre, Sault Ste Marie, ON P6A 2E5, Canada.
Entomology and Nematology Department, University of Florida, Bldg 970, Natural Area Drive, Gainesville, FL 32611, USA.
J Gen Virol. 2006 Jun;87(Pt 6):1477-1489. doi: 10.1099/vir.0.81727-0.
Genomic comparison of Neodiprion sertifer nucleopolyhedrovirus (NeseNPV) and Neodiprion lecontei nucleopolyhedrovirus (NeleNPV) showed that the hymenopteran baculoviruses had features in common and were distinct from other, fully sequenced lepidopteran and dipteran baculoviruses. Their genomes were small in size (86,462 and 81,755 bp, respectively), had low G+C contents (33.8 and 33.3 mol%, respectively) and contained fewer open reading frames (ORFs) (90 and 89, respectively) than other baculoviruses. They shared 69 ORFs (48.6% mean amino acid identity overall), 43 of which were previously identified baculovirus homologues. The remaining shared ORFs could be common to other baculoviruses, but low amino acid identities precluded identifying them as such. Some may also be unique to hymenopteran baculoviruses. These included a trypsin-like protease, a zinc-finger protein, regulator of chromosome condensation proteins, a densovirus capsid-like protein and a phosphotransferase. Structural analysis, the presence of conserved domains and phylogenetic studies suggested that some of these ORFs may be functional and could have been transferred horizontally from an insect host. ORFs found only in NeseNPV and NeleNPV may play a role in host specificity and/or tissue tropism, as hymenopteran baculoviruses are restricted to the midgut. The genomes were basically collinear, but contained non-syntenic regions (NSRs) with large numbers of repeats between their polyhedrin and dbp genes. They differed from each other in the number of ORFs and the G+C content of their NSRs and the presence of homologous regions in the NeseNPV genome. NeleNPV also had a short inversion relative to NeseNPV. NeseNPV contained 21 ORFs not found in NeleNPV and NeleNPV had 20 ORFs not found in NeseNPV.
黄杉毒蛾核多角体病毒（NeseNPV）和红头毒蛾核多角体病毒（NeleNPV）的基因组比较表明，膜翅目杆状病毒具有共同特征，且与其他已完成全序列测序的鳞翅目和双翅目杆状病毒不同。它们的基因组规模较小（分别为86,462和81,755碱基对），G+C含量较低（分别为33.8和33.3摩尔%），与其他杆状病毒相比，开放阅读框（ORF）数量较少（分别为90和89个）。它们共有69个ORF（总体平均氨基酸同一性为48.6%），其中43个是先前鉴定出的杆状病毒同源物。其余共享的ORF可能为其他杆状病毒所共有，但由于氨基酸同一性较低，无法将它们鉴定为此类。有些ORF也可能是膜翅目杆状病毒所特有的。这些包括一种类胰蛋白酶、一种锌指蛋白、染色体凝聚调节蛋白、一种浓核病毒衣壳样蛋白和一种磷酸转移酶。结构分析、保守结构域的存在以及系统发育研究表明，其中一些ORF可能具有功能，并且可能是从昆虫宿主水平转移而来的。仅在NeseNPV和NeleNPV中发现的ORF可能在宿主特异性和/或组织嗜性中发挥作用，因为膜翅目杆状病毒局限于中肠。这些基因组基本共线，但在其多角体蛋白和dbp基因之间包含具有大量重复序列的非共线区域（NSR）。它们在ORF数量、NSR的G+C含量以及NeseNPV基因组中同源区域的存在方面彼此不同。相对于NeseNPV，NeleNPV还存在一个短倒位。NeseNPV包含21个在NeleNPV中未发现的ORF，而NeleNPV有20个在NeseNPV中未发现的ORF。
