Molin G, Andersson R, Ahrné S, Lönner C, Marklinder I, Johansson M L, Jeppsson B, Bengmark S
Food- and Environmental Microbiology, Chemical Center, Lund, Sweden.
Antonie Van Leeuwenhoek. 1992 Apr;61(3):167-73. doi: 10.1007/BF00584223.
Rats were fed with freeze-dried oatmeal soup fermented by six different Lactobacillus strains from rat and man; the formula is intended for enteral feeding. The serum cholesterol levels after 10 d were lower for rats eating oatmeal as compared to a commercial product, Biosorb Sond. Colonizing ability of the administered strains were evaluated in vivo. Only Lactobacillus reuteri R21c were able to, effectively, colonizing the mucosa; it represented about 30% of the Lactobacillus population 24 d after termination of the administration. L. reuteri R21c was easily recognized by the ability to produce a yellow pigment on agar plates. The identity was confirmed by carbohydrate fermentations (API 50CH), plasmid pattern and endonuclease restriction analysis of the chromosomal DNA.
用来自大鼠和人类的六种不同乳酸杆菌菌株发酵的冻干燕麦汤喂养大鼠;该配方用于肠内喂养。与商业产品Biosorb Sond相比,食用燕麦片的大鼠在10天后血清胆固醇水平较低。在体内评估了所施用菌株的定殖能力。只有罗伊氏乳杆菌R21c能够有效地定殖于黏膜;在给药终止后24天,它占乳酸杆菌菌群的约30%。罗伊氏乳杆菌R21c通过在琼脂平板上产生黄色色素的能力很容易识别。通过碳水化合物发酵(API 50CH)、质粒图谱和染色体DNA的内切酶限制性分析确认了其身份。