用天然或重组肺炎链球菌神经氨酸酶进行免疫接种可在栗鼠中耳炎模型中提供保护。
Immunization with native or recombinant Streptococcus pneumoniae neuraminidase affords protection in the chinchilla otitis media model.
作者信息
Long J P, Tong H H, DeMaria T F
机构信息
Division of Otologic Research, College of Medicine and Public Health, The Ohio State University, Room 4331, Cramblett Hall, 456 W. 10th Ave., Columbus, OH 43210, USA.
出版信息
Infect Immun. 2004 Jul;72(7):4309-13. doi: 10.1128/IAI.72.7.4309-4313.2004.
Abstract
Streptococcus pneumoniae neuraminidase has been implicated as a virulence factor in the pathogenesis of pneumococcal otitis media. In this study, native neuraminidase was partially purified from cultures of S. pneumoniae by serial chromatography with DEAE-Sepharose and Sephacryl S-200. Recombinant neuraminidase, a 3,038-bp fragment of the neuraminidase A (nanA) gene, was cloned into the pET-28b vector and then expressed at high levels in Escherichia coli. Chinchillas were immunized subcutaneously with either the gel-purified native or recombinant neuraminidase, and all responded with elevated titers of antineuraminidase antibody in serum. Immunization with neuraminidase resulted in a significant reduction in nasopharyngeal colonization as well as in the incidence of otitis media with effusion. These data demonstrate for the first time that neuraminidase affords protection against S. pneumoniae nasopharyngeal colonization and experimental otitis media.
摘要
肺炎链球菌神经氨酸酶被认为是肺炎球菌性中耳炎发病机制中的一种毒力因子。在本研究中，通过用DEAE-琼脂糖和Sephacryl S-200进行连续层析，从肺炎链球菌培养物中部分纯化了天然神经氨酸酶。将重组神经氨酸酶（神经氨酸酶A（nanA）基因的一个3038 bp片段）克隆到pET-28b载体中，然后在大肠杆菌中高水平表达。用凝胶纯化的天然或重组神经氨酸酶对龙猫进行皮下免疫，所有动物血清中的抗神经氨酸酶抗体滴度均升高。用神经氨酸酶免疫可显著减少鼻咽部定植以及渗出性中耳炎的发病率。这些数据首次证明神经氨酸酶可提供针对肺炎链球菌鼻咽部定植和实验性中耳炎的保护作用。
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