耐万古霉素粪肠球菌的胞质肽聚糖前体以乳酸盐结尾。
The cytoplasmic peptidoglycan precursor of vancomycin-resistant Enterococcus faecalis terminates in lactate.
作者信息
Handwerger S, Pucci M J, Volk K J, Liu J, Lee M S
机构信息
Division of Infectious Diseases, Beth Israel Medical Center, Mount Sinai School of Medicine, New York, New York 10003.
出版信息
J Bacteriol. 1992 Sep;174(18):5982-4. doi: 10.1128/jb.174.18.5982-5984.1992.
Abstract
Vancomycin resistance plasmids in enterococci carry the genes vanH and vanA, which encode enzymes catalyzing, respectively, the reduction of 2-keto acids to 2-D-hydroxy acids and the addition of D-hydroxy acids to D-alanine. It has therefore been postulated that resistant cells produce peptidoglycan precursors that terminate in the depsipeptide D-alanine-2-D-hydroxy acid rather than the dipeptide D-alanine-D-alanine, thus preventing vancomycin binding (M. Arthur, C. Molinas, T. D. H. Bugg, G. D. Wright, C. T. Walsh, and P. Courvalin, Antimicrob. Agents Chemother. 36:867-869, 1992). In the present work, a cytoplasmic peptidoglycan precursor was isolated from vancomycin-resistant Enterococcus faecalis and analyzed by mass spectrometry, which suggested the structure UDP-N-acetyl-muramyl-L-Ala-D-Glu-L-Lys-D-Ala-D-lactate.
摘要
肠球菌中的万古霉素耐药质粒携带vanH和vanA基因，这两个基因分别编码催化2-酮酸还原为2-D-羟基酸以及将D-羟基酸添加到D-丙氨酸的酶。因此据推测，耐药细胞产生的肽聚糖前体以depsi肽D-丙氨酸-2-D-羟基酸而非二肽D-丙氨酸-D-丙氨酸结尾，从而阻止万古霉素结合（M. 亚瑟、C. 莫利纳斯、T. D. H. 巴格、G. D. 赖特、C. T. 沃尔什和P. 库尔瓦林，《抗菌剂与化疗》36:867 - 869，1992年）。在本研究中，从耐万古霉素的粪肠球菌中分离出一种细胞质肽聚糖前体，并通过质谱分析，结果表明其结构为UDP-N-乙酰-胞壁酰-L-丙氨酸-D-谷氨酸-L-赖氨酸-D-丙氨酸-D-乳酸。
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