角鲨烯对异丙肾上腺素诱导的大鼠心肌梗死组织防御系统的影响。

Effect of squalene on tissue defense system in isoproterenol-induced myocardial infarction in rats.

作者信息

Sabeena Farvin K H, Anandan R, Kumar S Hari Senthil, Shiny K S, Sankar T V, Thankappan T K

机构信息

Biochemistry and Nutrition Division, Central Institute of Fisheries Technology, Matsyapuri P.O., Cochin-682 029, India.

出版信息

Pharmacol Res. 2004 Sep;50(3):231-6. doi: 10.1016/j.phrs.2004.03.004.

DOI:10.1016/j.phrs.2004.03.004

PMID:15225664

Abstract

This study was designed to examine the effects of squalene on tissue antioxidant status in isoproterenol-induced myocardial infarction in male albino rats. Levels of diagnostic marker enzymes [alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), and creatine phosphokinase (CPK)] in plasma, lipid peroxides, reduced glutathione, and the activities of glutathione-dependent antioxidant enzymes [glutathione peroxidase (GPx) and glutathione-S-transferase (GST)] and antiperoxidative enzymes [catalase (CAT) and superoxide dismutase (SOD)] in the heart tissue of experimental groups of rats were determined. The prior administration of squalene at 2% level along with feed for 45 days significantly prevented the isoproterenol-induced elevation in the levels of diagnostic marker enzymes in plasma of experimental rats. Squalene also exerted an antioxidant effect against isoproterenol-induced myocardial infarction by blocking the induction of lipid peroxidation. A tendency to prevent the isoproterenol-induced alterations in the level of reduced glutathione and in the activities of glutathione-dependent antioxidant enzymes and antiperoxidative enzymes was also observed. The cardioprotective effect of squalene might be ascribable to its antioxidant property and membrane stabilizing action.

摘要

本研究旨在探讨角鲨烯对异丙肾上腺素诱导的雄性白化大鼠心肌梗死组织抗氧化状态的影响。测定了实验组大鼠血浆中诊断标志物酶[丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）、乳酸脱氢酶（LDH）和肌酸磷酸激酶（CPK）]的水平、脂质过氧化物、还原型谷胱甘肽以及心脏组织中谷胱甘肽依赖性抗氧化酶[谷胱甘肽过氧化物酶（GPx）和谷胱甘肽-S-转移酶（GST）]及抗过氧化酶[过氧化氢酶（CAT）和超氧化物歧化酶（SOD）]的活性。预先以2%的水平将角鲨烯与饲料一起投喂45天，可显著防止异丙肾上腺素诱导的实验大鼠血浆中诊断标志物酶水平升高。角鲨烯还通过阻止脂质过氧化的诱导，对异丙肾上腺素诱导的心肌梗死发挥抗氧化作用。还观察到角鲨烯有防止异丙肾上腺素诱导的还原型谷胱甘肽水平以及谷胱甘肽依赖性抗氧化酶和抗过氧化酶活性改变的趋势。角鲨烯的心脏保护作用可能归因于其抗氧化特性和膜稳定作用。