Center for Biofilm Engineering, Montana State University, Bozeman, MT, USA.
J Appl Microbiol. 2012 May;112(5):998-1006. doi: 10.1111/j.1365-2672.2012.05264.x. Epub 2012 Mar 13.
The goal of this investigation was to develop an in vitro, polymicrobial, wound biofilm capable of supporting the growth of bacteria with variable oxygen requirements.
The strict anaerobe Clostridium perfringens was isolated by cultivating wound homogenates using the drip-flow reactor (DFR), and a three-species biofilm model was established using methicillin-resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa and Cl. perfringens in the colony-drip-flow reactor model. Plate counts revealed that MRSA, Ps. aeruginosa and Cl. perfringens grew to 7·39 ± 0·45, 10·22 ± 0·22 and 7·13 ± 0·77 log CFU per membrane, respectively. The three-species model was employed to evaluate the efficacy of two antimicrobial dressings, Curity™ AMD and Acticoat™, compared to sterile gauze controls. Microbial growth on Curity™ AMD and gauze was not significantly different, for any species, whereas Acticoat™ was found to significantly reduce growth for all three species.
Using the colony-DFR, a three-species biofilm was successfully grown, and the biofilms displayed a unique structure consisting of distinct layers that appeared to be inhabited exclusively or predominantly by a single species.
The primary accomplishment of this study was the isolation and growth of an obligate anaerobe in an in vitro model without establishing an artificially anaerobic environment.
本研究旨在开发一种体外多微生物伤口生物膜，该生物膜能够支持需氧要求不同的细菌生长。
通过使用滴流反应器（DFR）培养伤口匀浆，从伤口中分离出严格厌氧菌产气荚膜梭菌（Clostridium perfringens），并在集落滴流反应器模型中使用耐甲氧西林金黄色葡萄球菌（MRSA）、铜绿假单胞菌（Pseudomonas aeruginosa）和 Cl. perfringens 建立了三物种生物膜模型。平板计数显示，MRSA、Ps. aeruginosa 和 Cl. perfringens 在膜上的生长分别达到 7.39 ± 0.45、10.22 ± 0.22 和 7.13 ± 0.77 log CFU。将三物种模型用于评估两种抗菌敷料 Curity™ AMD 和 Acticoat™与无菌纱布对照相比的疗效。对于任何一种物种，Curity™ AMD 和纱布上的微生物生长没有显著差异，而 Acticoat™被发现显著减少了所有三种物种的生长。
使用集落-DFR，成功地生长了三物种生物膜，并且生物膜显示出独特的结构，由明显的层组成，这些层似乎仅或主要由单一物种居住。
本研究的主要成就是在没有建立人工厌氧环境的情况下，从体外模型中分离出并生长出一种专性厌氧菌。
