Yap Melvyn W, Nisole Sébastien, Lynch Clare, Stoye Jonathan P
Division of Virology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, United Kingdom.
Proc Natl Acad Sci U S A. 2004 Jul 20;101(29):10786-91. doi: 10.1073/pnas.0402876101. Epub 2004 Jul 12.
Replication of HIV-1 and N-tropic murine leukemia virus (N-MLV) is restricted in a number of different primate cells. In some cell lines, cross-saturation experiments suggest that the two viruses are interacting with the same restriction factor. Recently, Trim5alpha protein from rhesus monkey was found to restrict HIV-1. We have confirmed this result and have shown that Trim5alpha from two African green monkey cell lines, Vero and CV-1, also restricts HIV-1. In addition, we show that human, rhesus, and African green monkey Trim5alpha can restrict N-MLV. By using a panel of MLV capsid mutants, subtle differences in the anti-MLV activity were identified among the different primate Trim5alpha cDNAs. Trim1 isolated from humans and green monkeys was also found to restrict N-MLV. We hypothesize that the Trim family of proteins plays a widespread role in innate immunity to viral infection.
HIV-1和N-嗜性鼠白血病病毒（N-MLV）在许多不同的灵长类细胞中复制受到限制。在一些细胞系中，交叉饱和实验表明这两种病毒与相同的限制因子相互作用。最近，发现恒河猴的Trim5α蛋白可限制HIV-1。我们证实了这一结果，并表明来自两种非洲绿猴细胞系Vero和CV-1的Trim5α也能限制HIV-1。此外，我们还表明人类、恒河猴和非洲绿猴的Trim5α均可限制N-MLV。通过使用一组MLV衣壳突变体，在不同灵长类Trim5α cDNA之间鉴定出抗MLV活性的细微差异。从人类和绿猴中分离出的Trim1也被发现可限制N-MLV。我们推测Trim蛋白家族在病毒感染的先天免疫中发挥广泛作用。
