Ivanova Elena P, Nedashkovskaya Olga I, Sawabe Tomoo, Zhukova Natalia V, Frolova Galina M, Nicolau Dan V, Mikhailov Valery V, Bowman John P
Pacific Institute of Bioorganic Chemistry of the Far-Eastern Branch of the Russian Academy of Sciences, 690022 Vladivostok, Pr. 100 Let Vladivostoku 159, Russia.
Industrial Research Institute, Swinburne University of Technology, PO Box 218, Hawthorn, Vic 3122, Australia.
Int J Syst Evol Microbiol. 2004 Jul;54(Pt 4):1089-1093. doi: 10.1099/ijs.0.02992-0.
Four marine bacterial strains, designated KMM 3587T, KMM 3586, KMM 3821 and KMM 3822, were isolated from the sipuncula Phascolosoma japonicum, a common inhabitant of Troitza Bay in the Gulf of Peter the Great (Sea of Japan region), and from an unidentified hydrocoral species collected in Makarov Bay (Iturup Islands), Kuril Islands, North-West Pacific Ocean. The strains were characterized to clarify their taxonomic position. 16S rRNA gene sequences of KMM 3587T and KMM 3586 indicated 99% similarity to Shewanella colwelliana. Despite such a high level of 16S rRNA gene sequence similarity, DNA-DNA hybridization experiments demonstrated only 45-52% binding with DNA of S. colwelliana ATCC 39565T. The DNA G+C contents of the novel strains were 45 mol% and the shared level of DNA hybridization was conspecific (81-97%), indicating that they represent a single genospecies. The novel strains were mesophilic (able to grow at 10-34 degrees C), neutrophilic and haemolytic, and able to degrade gelatin, casein and Tween 20, 40 and 80, but not starch, agar, elastin, alginate or chitin. The major fatty acids were i13 : 0, i15 : 0, 16 : 0, 16 : 1omega7 and 17 : 1omega8 (68.9% of total). The major isoprenoid quinones were Q7 (47-62%) and Q8 (26-47%). Eicosapentaenoic acid was produced in minor amounts. Based on these data, the strains are assigned to a novel species, Shewanella affinis sp. nov. (type strain KMM 3587T=CIP 107703T=ATCC BAA-642T).
从多毛纲动物日本革囊星虫（一种生活在大彼得湾（日本海区域）特罗伊察湾的常见生物）以及从西北太平洋千岛群岛伊图鲁普岛马卡罗夫湾采集的一种未鉴定的水螅珊瑚中分离出了四株海洋细菌菌株，分别命名为KMM 3587T、KMM 3586、KMM 3821和KMM 3822。对这些菌株进行了表征以明确它们的分类地位。KMM 3587T和KMM 3586的16S rRNA基因序列显示与科尔韦尔希瓦氏菌有99%的相似性。尽管16S rRNA基因序列相似度如此之高，但DNA - DNA杂交实验表明与科尔韦尔希瓦氏菌ATCC 39565T的DNA仅有45 - 52%的结合。新菌株的DNA G + C含量为45 mol%，DNA杂交的共享水平为同种（81 - 97%），表明它们代表一个单一的基因种。新菌株为嗜温菌（能够在10 - 34摄氏度生长）、嗜中性且具有溶血活性，并且能够降解明胶、酪蛋白以及吐温20、40和80，但不能降解淀粉、琼脂、弹性蛋白、藻酸盐或几丁质。主要脂肪酸为i13 : 0、i15 : 0、16 : 0、16 : 1ω7和17 : 1ω8（占总量的68.9%）。主要类异戊二烯醌为Q7（47 - 62%）和Q8（26 - 47%）。二十碳五烯酸的产量较少。基于这些数据，这些菌株被归为一个新物种，亲近希瓦氏菌（Shewanella affinis sp. nov.）（模式菌株KMM 3587T = CIP 107703T = ATCC BAA - 642T）。
