Wagner-Döbler Irene, Rheims Holger, Felske Andreas, El-Ghezal Aymen, Flade-Schröder Dirk, Laatsch Hartmut, Lang Siegmund, Pukall Rüdiger, Tindall Brian J
GBF - Gesellschaft für Biotechnologische Forschung, D-38124 Braunschweig, Germany.
Technical University of Braunschweig, D-38106 Braunschweig, Germany.
Int J Syst Evol Microbiol. 2004 Jul;54(Pt 4):1177-1184. doi: 10.1099/ijs.0.02850-0.
A water sample from the North Sea was used to isolate the abundant heterotrophic bacteria that are able to grow on complex marine media. Isolation was by serial dilution and spread plating. Phylogenetic analysis of nearly complete 16S rRNA gene sequences revealed that one of the strains, HEL-45T, had 97.4% sequence similarity to Sulfitobacter mediterraneus and 96.5 % sequence similarity to Staleya guttiformis. Strain HEL-45T is a Gram-negative, non-motile rod and obligate aerobe and requires sodium and 1-7% sea salts for growth. It contains storage granules and does not produce bacteriochlorophyll. Optimal growth temperatures are 25-30 degrees C. The DNA base composition (G+C content) is 60.1 mol%. Strain HEL-45T has Q10 as the dominant respiratory quinone. The major polar lipids are phosphatidyl glycerol, diphosphatidyl glycerol, phosphatidyl choline, phosphatidyl ethanolamine and an aminolipid. The fatty acids comprise 18 : 1omega7c, 18 : 0, 16 : 1omega7c, 16 : 0, 3-OH 10 : 0, 3-OH 12 : 1 (or 3-oxo 12 : 0) and traces of an 18 : 2 fatty acid. Among the hydroxylated fatty acids only 3-OH 12 : 1 (or 3-oxo 12 : 0) appears to be amide linked, whereas 3-OH 10 : 0 appears to be ester linked. The minor fatty acid components (between 1 and 7%) allow three subgroups to be distinguished in the Sulfitobacter/Staleya clade, placing HEL-45T into a separate lineage characterized by the presence of 3-OH 12 : 1 (or 3-oxo 12 : 0) and both ester- and amide-linked 16 : 1omega7c phospholipids. HEL-45T produces indole and derivatives thereof, several cyclic dipeptides and thryptanthrin. Phylogenetic analysis of 16S rRNA gene sequences and chemotaxonomic data support the description of a new genus and species, to include Oceanibulbus indolifex gen. nov., sp. nov., with the type strain HEL-45T (=DSM 14862T=NCIMB 13983T).
从北海采集的一份水样被用于分离能够在复杂海洋培养基上生长的丰富的异养细菌。通过连续稀释和平板涂布进行分离。对近乎完整的16S rRNA基因序列进行系统发育分析表明，其中一个菌株HEL-45T与地中海硫杆菌的序列相似性为97.4%，与球形斯塔利氏菌的序列相似性为96.5%。菌株HEL-45T是革兰氏阴性、不运动的杆菌，专性需氧菌，生长需要钠和1 - 7%的海盐。它含有储存颗粒，不产生细菌叶绿素。最适生长温度为25 - 30摄氏度。DNA碱基组成（G + C含量）为60.1 mol%。菌株HEL-45T以Q10作为主要呼吸醌。主要极性脂类为磷脂酰甘油、双磷脂酰甘油、磷脂酰胆碱、磷脂酰乙醇胺和一种氨基脂。脂肪酸包括18 : 1ω7c、18 : 0、16 : 1ω7c、16 : 0、3 - OH 10 : 0、3 - OH 12 : 1（或3 - 氧代12 : 0）以及痕量的18 : 2脂肪酸。在羟基化脂肪酸中，只有3 - OH 12 : 1（或3 - 氧代12 : 0）似乎是酰胺连接的，而3 - OH 10 : 0似乎是酯连接的。次要脂肪酸成分（1%至7%）使得在硫杆菌/斯塔利氏菌分支中能够区分出三个亚组，将HEL-45T置于一个以存在3 - OH 12 : 1（或3 - 氧代12 : 0）以及酯连接和酰胺连接的16 : 1ω7c磷脂为特征的单独谱系中。HEL-45T产生吲哚及其衍生物、几种环二肽和色胺酮。16S rRNA基因序列的系统发育分析和化学分类数据支持描述一个新属和新种，即吲哚海洋杆菌属新属，新种，模式菌株为HEL-45T（=DSM 14862T = NCIMB 13983T）。
