Cui Xiaolong, Schumann Peter, Stackebrandt Erko, Kroppenstedt Reiner M, Pukall Rüdiger, Xu Lihua, Rohde Manfred, Jiang Chenglin
The Key Laboratory for Microbial Resources of Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, P. R. China.
DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen, Mascheroder Weg 1b, D-38124 Braunschweig, Germany.
Int J Syst Evol Microbiol. 2004 Jul;54(Pt 4):1287-1293. doi: 10.1099/ijs.0.03046-0.
Strain XLG9A10.2T was isolated from an alkaline salt marsh soil in western China. 16S rRNA gene sequence analysis indicated that strain XLG9A10.2T constitutes a distinct lineage within the family Promicromonosporaceae, sharing 94.8-95.1% gene similarity with members of the genus Promicromonospora and 94.4-95.7% similarity with those of Xylanimonas and related genera. The general colony and cell morphology of strain XLG9A10.2T is similar to that of members of Promicromonospora, but differs from members of the genus Xylanimonas in forming a well-developed branching mycelium and production of coccoid spores. Strain XLG9A10.2T shows the peptidoglycan type A4alpha (L-lys<--L-thr<--D-Glu), contains glucose, mannose and galactose as whole cell sugars and has MK-9(H4) and MK-9(H6) as major menaquinones, while phospholipids are phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol, three unidentified phospholipids and one unidentified glycolipid. The DNA base composition is 71.9 mol% G+C. On the basis of morphological, chemotaxonomic, metabolic and phylogenetic differences from other species of Promicromonosporaceae, a new genus and species, Myceligenerans xiligouense gen. nov., sp. nov., is proposed. The type strain is XLG9A10.2T (=DSM 15700T=CGMCC 1.3458T.)
菌株XLG9A10.2T是从中国西部的碱性盐沼土壤中分离得到的。16S rRNA基因序列分析表明，菌株XLG9A10.2T在原小单孢菌科内构成一个独特的谱系，与原小单孢菌属成员的基因相似性为94.8 - 95.1%，与木单孢菌属及相关属的相似性为94.4 - 95.7%。菌株XLG9A10.2T的一般菌落和细胞形态与原小单孢菌属成员相似，但与木单孢菌属成员不同，它能形成发育良好的分支菌丝体并产生球状孢子。菌株XLG9A10.2T显示出A4α型肽聚糖（L-赖氨酸←L-苏氨酸←D-谷氨酸），全细胞糖包含葡萄糖、甘露糖和半乳糖，主要甲基萘醌为MK-9(H4)和MK-9(H6)，而磷脂为磷脂酰甘油、二磷脂酰甘油、磷脂酰肌醇、三种未鉴定的磷脂和一种未鉴定的糖脂。DNA碱基组成为71.9 mol% G+C。基于与原小单孢菌科其他物种在形态、化学分类、代谢和系统发育方面的差异，提出了一个新属和新种，即西李沟生丝菌属（Myceligenerans xiligouense），新属，新种。模式菌株为XLG9A10.2T（=DSM 15700T=CGMCC 1.3458T）
