Maurer Hans H
Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical Pharmacology and Toxicology, University of Saarland, 66421 Homburg (Saar), Germany.
Anal Bioanal Chem. 2005 Jan;381(1):110-8. doi: 10.1007/s00216-004-2774-z. Epub 2004 Aug 12.
This paper reviews procedures for quantification of drugs in the biosamples blood, plasma, serum, or oral fluid (saliva, etc.) using liquid chromatography coupled with single-stage or tandem mass spectrometry (LC-MS, LC-MS-MS). Such procedures are important prerequisites for competent toxicological judgment and consultation in clinical and forensic toxicology. They cover blood (plasma, serum) analysis of amphetamines and related designer drugs, anesthetics, anticonvulsants, benzodiazepines, opioids, serotonergic drugs, tricyclic antidepressants, neuroleptics, antihistamines, beta-blockers, muscle relaxants, and sulfonylurea-type antidiabetics, and oral fluid analysis of amphetamines and related designer drugs, cocaine, benzoylecgonine, codeine, morphine, enantiomers of methadone and its main metabolite 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP), the nicotine metabolites cotinine and hydroxycotinine, and finally risperidone and its metabolite 9-hydroxyrisperidone. Basic information on the procedures is given in two tables and an example of quantification is illustrated in two figures. The pros and cons of such LC-MS procedures including sample work-up and ion suppression effects are critically discussed.
本文综述了使用液相色谱联用单级或串联质谱（LC-MS、LC-MS-MS）对生物样本血液、血浆、血清或口腔液（唾液等）中的药物进行定量的方法。这些方法是临床和法医毒理学中进行有效毒理学判断和咨询的重要前提条件。它们涵盖了对苯丙胺类及相关设计药物、麻醉剂、抗惊厥药、苯二氮䓬类、阿片类药物、血清素能药物、三环类抗抑郁药、抗精神病药、抗组胺药、β受体阻滞剂、肌肉松弛剂和磺酰脲类抗糖尿病药物的血液（血浆、血清）分析，以及对苯丙胺类及相关设计药物、可卡因、苯甲酰芽子碱、可待因、吗啡、美沙酮对映体及其主要代谢物2-亚乙基-1,5-二甲基-3,3-二苯基吡咯烷（EDDP）、尼古丁代谢物可替宁和羟基可替宁，以及最后利培酮及其代谢物9-羟基利培酮的口腔液分析。方法的基本信息列于两个表格中，定量示例在两个图中展示。本文对这类LC-MS方法的优缺点，包括样品处理和离子抑制效应进行了批判性讨论。
