Weiser M G
Department of Pathology, College of Veterinary Medicine, Colorado State University, Ft. Collins, Colorado 80523.
Vet Clin Pathol. 1983;12(2):25-32. doi: 10.1111/j.1939-165x.1983.tb00611.x.
An automated, multi-channel blood cell counting system (S-Plus) was compared to a reference counting system using blood samples from 187 animals of four species. The standard red cell bath aperture current of 150 volts (V) was used during analysis of 75% of the samples. At this setting, all samples with a Mean Corpuscular Volume (MCV) greater than 50 fl had accurate erythrocyte counts. As the MCV decreased below 50 fl, the severity of false low erythrocyte counts and false high MCV values increased. The remaining 25% of samples were analyzed with the red cell bath aperture current increased to 200 V. At this setting, only 5% or less of erythrocytes from animals with normal MCV values(>36 fl)were below the erythrocyte threshold. The red cell distribution width values provided by the S-Plus indicated that equine and bovine erythrocytes have greater anisocytosis than canine and feline erythrocytes. Leukocyte counts were significantly lower on the S-Plus (p<0.01). Canine and equine samples most frequently had platelet size distribution within the S-Plus platelet counting threshold window. Electronic whole blood platelet counting appeared unsatisfactory in cats due to large platelet size and erythrocyte-platelet size overlap. Small platelet size in cattle indicated that further modifications of the red cell bath aperture current would be required to count and size platelets in this species. Following electronic modifications, this state-of-the-art system appears adaptable to hematologic profiling in most species.
将一种自动化多通道血细胞计数系统（S-Plus）与一种参考计数系统进行比较，使用来自四个物种的187只动物的血样。在分析75%的样本时，使用150伏（V）的标准红细胞浴孔电流。在此设置下，所有平均红细胞体积（MCV）大于50飞升的样本都有准确的红细胞计数。随着MCV降至50飞升以下，红细胞计数假性偏低和MCV值假性偏高的严重程度增加。其余25%的样本在红细胞浴孔电流增加到200伏的情况下进行分析。在此设置下，MCV值正常（>36飞升）的动物中，只有5%或更少的红细胞低于红细胞阈值。S-Plus提供的红细胞分布宽度值表明，马和牛的红细胞比犬和猫的红细胞有更大的异质性。S-Plus上的白细胞计数显著更低（p<0.01）。犬和马的样本在S-Plus血小板计数阈值窗口内最常出现血小板大小分布。由于血小板体积大且红细胞与血小板大小重叠，电子全血血小板计数在猫身上似乎不太令人满意。牛的血小板体积小表明，需要进一步修改红细胞浴孔电流才能对该物种的血小板进行计数和测量大小。经过电子修改后，这种先进的系统似乎适用于大多数物种的血液学分析。
