Abu El-Asrar Ahmed M, Struyf Sofie, Descamps Francis J, Al-Obeidan Saleh A, Proost Paul, Van Damme Jo, Opdenakker Ghislain, Geboes Karel
Department of Ophthalmology, College of Medicine, King Saud University, Riyadh, Saudi Arabia.
Am J Ophthalmol. 2004 Sep;138(3):401-11. doi: 10.1016/j.ajo.2004.04.046.
To investigate the involvement of the chemokines CXCL10/IP-10, CXCL11/I-TAC, CXCL8/1L-8, CXCL6/GCP-2, CCL3/MIP-1alpha, and CCL18/PARC, and gelatinases A and B in uveitis.
Prospective, experimental, case-control study.
Aqueous humor samples from 30 patients with active uveitis, and 14 control patients and paired serum samples were assayed for chemokines with specific enzyme-linked immunosorbent assays (ELISAs) and for gelatinase levels by quantitative zymography.
In control AH, none of the chemokines was detected. Gelatinase A was detected in all samples, and gelatinase B was detected in only one sample. In patients with uveitis, IP-10 was detected in all AH samples, whereas I-TAC, IL-8, GCP-2, MIP-1alpha, and PARC were detected in three, 16, six, two, and 12 samples, respectively. IP-10 levels were significantly higher in AH samples than those of serum (P =.006). Gelatinase A was detected in 29 AH samples and gelatinase B was detected in 26 samples. Gelatinase A levels were significantly higher in AH samples from patients than those of controls (P <.0001). In 11 AH samples, gelatinase B was detected in complex with lipocalin (NGAL). Disease activity correlated significantly with the levels of IP-10 (r =.627; P <.0001), gelatinase A (r =.508; P =.002), gelatinase B (r =.685; P <.0001), and NGAL-gelatinase B complex (r =.595; P <.0001).
These data suggest a pathogenic role of the T lymphocyte chemoattractant IP-10 and gelatinases in the recruitment and activity of T cells into the eye in patients with uveitis and in the pathogenesis of uveitis.
研究趋化因子CXCL10/IP-10、CXCL11/I-TAC、CXCL8/IL-8、CXCL6/GCP-2、CCL3/MIP-1α和CCL18/PARC以及明胶酶A和B在葡萄膜炎中的作用。
前瞻性、实验性、病例对照研究。
采用特异性酶联免疫吸附测定（ELISA）法检测30例活动性葡萄膜炎患者和14例对照患者的房水样本以及配对血清样本中的趋化因子，并通过定量酶谱法检测明胶酶水平。
在对照房水中，未检测到任何趋化因子。所有样本中均检测到明胶酶A，仅在一个样本中检测到明胶酶B。在葡萄膜炎患者中，所有房水样本均检测到IP-10，而I-TAC、IL-8、GCP-2、MIP-1α和PARC分别在3份、16份、6份、2份和12份样本中检测到。房水样本中IP-10水平显著高于血清（P = 0.006）。29份房水样本中检测到明胶酶A，26份样本中检测到明胶酶B。患者房水样本中明胶酶A水平显著高于对照组（P < 0.0001）。在11份房水样本中，检测到明胶酶B与脂质运载蛋白（NGAL）形成复合物。疾病活动度与IP-10（r = 0.627；P < 0.0001）、明胶酶A（r = 0.508；P = 0.002）、明胶酶B（r = 0.685；P < 0.0001）和NGAL-明胶酶B复合物（r = 0.595；P < 0.0001）水平显著相关。
这些数据表明，T淋巴细胞趋化因子IP-10和明胶酶在葡萄膜炎患者T细胞向眼内的募集和活性以及葡萄膜炎的发病机制中起致病作用。
