Kremer Kristin, Glynn Judith R, Lillebaek Troels, Niemann Stefan, Kurepina Natalia E, Kreiswirth Barry N, Bifani Pablo J, van Soolingen Dick
Mycobacteria Reference Unit, Diagnostic Laboratory for Infectious Diseases and Perinatal Screening, National Institute of Public Health and the Environment, P.O. Box 1, 3720 BA Bilthoven, The Netherlands.
J Clin Microbiol. 2004 Sep;42(9):4040-9. doi: 10.1128/JCM.42.9.4040-4049.2004.
Mycobacterium tuberculosis Beijing genotype strains are highly prevalent in Asian countries and in the territory of the former Soviet Union. They are increasingly reported in other areas of the world and are frequently associated with tuberculosis outbreaks and drug resistance. Beijing genotype strains, including W strains, have been characterized by their highly similar multicopy IS6110 restriction fragment length polymorphism (RFLP) patterns, deletion of spacers 1 to 34 in the direct repeat region (Beijing spoligotype), and insertion of IS6110 in the genomic dnaA-dnaN locus. In this study the suitability and comparability of these three genetic markers to identify members of the Beijing lineage were evaluated. In a well-characterized collection of 1,020 M. tuberculosis isolates representative of the IS6110 RFLP genotypes found in The Netherlands, strains of two clades had spoligotypes characteristic of the Beijing lineage. A set of 19 Beijing reference RFLP patterns was selected to retrieve all Beijing strains from the Dutch database. These reference patterns gave a sensitivity of 98.1% and a specificity of 99.7% for identifying Beijing strains (defined by spoligotyping) in an international database of 1,084 strains. The usefulness of the reference patterns was also assessed with large DNA fingerprint databases in two other European countries and for identification strains from the W lineage found in the United States. A standardized definition for the identification of M. tuberculosis strains belonging to the Beijing/W lineage, as described in this work, will facilitate further studies on the spread and characterization of this widespread genotype family of M. tuberculosis strains.
结核分枝杆菌北京基因型菌株在亚洲国家和前苏联地区高度流行。在世界其他地区也有越来越多的报道，并且经常与结核病暴发和耐药性相关。北京基因型菌株，包括W菌株，其特征在于具有高度相似的多拷贝IS6110限制性片段长度多态性（RFLP）模式、直接重复区域中间隔序列1至34的缺失（北京间隔寡核苷酸分型）以及IS6110在基因组dnaA - dnaN位点的插入。在本研究中，评估了这三种遗传标记物用于鉴定北京分支成员的适用性和可比性。在一组经过充分表征的1020株结核分枝杆菌分离株中，这些分离株代表了在荷兰发现的IS6110 RFLP基因型，其中两个进化枝的菌株具有北京分支特征性的间隔寡核苷酸分型。选择了一组19种北京参考RFLP模式，以便从荷兰数据库中检索所有北京菌株。在一个包含1084株菌株的国际数据库中，这些参考模式对鉴定北京菌株（由间隔寡核苷酸分型定义）的灵敏度为98.1%，特异性为99.7%。还利用另外两个欧洲国家的大型DNA指纹数据库评估了参考模式的实用性，并用于鉴定在美国发现的W分支菌株。如本文所述，对属于北京/W分支的结核分枝杆菌菌株进行鉴定的标准化定义将有助于进一步研究这种广泛分布的结核分枝杆菌基因型家族的传播和特征。
