氟代葡萄糖跨人红细胞膜交换的异头偏好性。19F核磁共振研究。
Anomeric preference of fluoroglucose exchange across human red-cell membranes. 19F-n.m.r. studies.
作者信息
Potts J R, Kuchel P W
机构信息
Department of Biochemistry, University of Sydney, N.S.W., Australia.
出版信息
Biochem J. 1992 Feb 1;281 ( Pt 3)(Pt 3):753-9. doi: 10.1042/bj2810753.
The rates of exchange across the human red-cell membrane of the alpha- and beta-anomers of the glucose derivative 3-fluoro-3-deoxy-D-glucose (3FG) were measured, under equilibrium-exchange conditions, using a 19F-n.m.r.-magnetization-exchange procedure. In experiments carried out over a range of 3FG concentrations (3.4-113 mM), the alpha-anomer was found to be transported with a smaller Km (greater apparent affinity) than the beta-anomer. In two experiments carried out at 34 and 37 degrees C the ratio (alpha/beta) of the Michaelis constants for exchange was 0.75 +/- 0.07 and 0.83 +/- 0.07 respectively and the Vmax for 3FG exchange was 28 +/- 3 and 33 +/- 3 mmol.s-1.litre of cells-1 respectively. In several experiments carried out at a single 3FG concentration (17 mM) and at 37 degrees C, using red cells from four individuals, the rate of exchange of the alpha-anomer across the membrane was significantly higher than that of the beta-anomer. The weighted mean value of the above-mentioned ratio was 0.79 +/- 0.07 for the four donors.
在平衡交换条件下，采用¹⁹F-核磁共振磁化交换程序，测定了葡萄糖衍生物3-氟-3-脱氧-D-葡萄糖（3FG）的α-和β-异头物跨人红细胞膜的交换速率。在一系列3FG浓度（3.4 - 113 mM）下进行的实验中，发现α-异头物的转运Km值（表观亲和力更高）比β-异头物小。在34℃和37℃进行的两项实验中，交换的米氏常数之比（α/β）分别为0.75±0.07和0.83±0.07，3FG交换的Vmax分别为28±3和33±3 mmol·s⁻¹·升细胞⁻¹。在37℃下，使用来自四个个体的红细胞，在单一3FG浓度（17 mM）下进行的几次实验中，α-异头物跨膜的交换速率明显高于β-异头物。四位供体上述比值的加权平均值为0.79±0.07。
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