在进行体外溶解试验的水样中微囊藻毒素-LR、微囊藻毒素-RR和微囊藻毒素-YR的分解情况。

Decomposition of microcystin-LR, microcystin-RR, and microcystin-YR in water samples submitted to in vitro dissolution tests.

作者信息

Moreno Isabel M, Maraver Judith, Aguete Esther C, Leao Manel, Gago-Martínez Ana, Cameán Ana M

机构信息

Nutrition, Food Chemistry, and Toxicology, Faculty of Pharmacy, University of Sevilla, C/Profesor García González 2, 41012 Sevilla, Spain.

出版信息

J Agric Food Chem. 2004 Sep 22;52(19):5933-8. doi: 10.1021/jf0489668.

DOI:10.1021/jf0489668

PMID:15366845

Abstract

The presence of cyanobacterial toxins (microcystins) in waters and food increases the risk of toxicity to animal and human health. These toxins can degrade in the human gastrointestinal tract before they are absorbed. To evaluate this possible degradation, water samples spiked with known concentrations of microcystins MC-LR, MC-RR, and MC-YR, which are the toxins most commonly produced by such toxic cyanobacteria as Microcystis aeruginosa, Oscillatoria spp., and Nostoc spp., were submitted to a dissolution test that used gastric and intestinal fluids according to U.S. Pharmacopeia conditions. HPLC with UV detection was used to determine the toxins before and after treatments. This study revealed enzymatic alterations in gastric conditions for all the toxins assayed. MC-RR was the toxin most affected: its range of inactivation was 49-64%. The percentage of degradation for MC-YR and MC-LR was around 30%. However, none was degraded by intestinal digestion.

摘要

水体和食物中蓝藻毒素（微囊藻毒素）的存在增加了对动物和人类健康产生毒性的风险。这些毒素在被吸收之前可在人体胃肠道中降解。为评估这种可能的降解情况，将添加了已知浓度微囊藻毒素MC-LR、MC-RR和MC-YR（这些是铜绿微囊藻、颤藻属和念珠藻属等有毒蓝藻最常产生的毒素）的水样按照美国药典条件进行使用胃液和肠液的溶解试验。采用带紫外检测的高效液相色谱法测定处理前后的毒素。本研究揭示了所检测的所有毒素在胃部条件下的酶促变化。MC-RR是受影响最大的毒素：其失活范围为49%-64%。MC-YR和MC-LR的降解百分比约为30%。然而，没有一种毒素能被肠道消化降解。