Zimmermann Philip, Hirsch-Hoffmann Matthias, Hennig Lars, Gruissem Wilhelm
Institute of Plant Sciences, Swiss Federal Institute of Technology and Zurich-Basel Plant Science Center, ETH Center, CH-8092 Zurich, Switzerland.
Plant Physiol. 2004 Sep;136(1):2621-32. doi: 10.1104/pp.104.046367.
High-throughput gene expression analysis has become a frequent and powerful research tool in biology. At present, however, few software applications have been developed for biologists to query large microarray gene expression databases using a Web-browser interface. We present GENEVESTIGATOR, a database and Web-browser data mining interface for Affymetrix GeneChip data. Users can query the database to retrieve the expression patterns of individual genes throughout chosen environmental conditions, growth stages, or organs. Reversely, mining tools allow users to identify genes specifically expressed during selected stresses, growth stages, or in particular organs. Using GENEVESTIGATOR, the gene expression profiles of more than 22,000 Arabidopsis genes can be obtained, including those of 10,600 currently uncharacterized genes. The objective of this software application is to direct gene functional discovery and design of new experiments by providing plant biologists with contextual information on the expression of genes. The database and analysis toolbox is available as a community resource at https://www.genevestigator.ethz.ch.
高通量基因表达分析已成为生物学中常用且强大的研究工具。然而目前，很少有软件应用程序供生物学家使用网络浏览器界面查询大型微阵列基因表达数据库。我们展示了GENEVESTIGATOR，这是一个用于Affymetrix基因芯片数据的数据库和网络浏览器数据挖掘界面。用户可以查询该数据库，以检索在选定的环境条件、生长阶段或器官中单个基因的表达模式。相反，挖掘工具允许用户识别在选定的胁迫、生长阶段或特定器官中特异性表达的基因。使用GENEVESTIGATOR，可以获得超过22000个拟南芥基因的基因表达谱，包括10600个目前未表征的基因。该软件应用程序的目标是通过为植物生物学家提供基因表达的背景信息来指导基因功能发现和新实验设计。该数据库和分析工具箱可作为社区资源在https://www.genevestigator.ethz.ch获取。
