一种可能为犬科动物所特有的高度重复DNA序列。

A highly repetitive DNA sequence possibly unique to canids.

作者信息

Minnick M F, Stillwell L C, Heineman J M, Stiegler G L

机构信息

Pacific Northwest Laboratories, Richland, WA 99352.

出版信息

Gene. 1992 Jan 15;110(2):235-8. doi: 10.1016/0378-1119(92)90654-8.

DOI:10.1016/0378-1119(92)90654-8

PMID:1537560

Abstract

A short interspersed nucleotide (nt) element (SINE) was cloned from the genomic DNA of the domestic dog, Canis familiaris. Southern-blot analysis of canine DNA digested with four restriction endonucleases indicated that the SINE is widely dispersed throughout the genome. Hybridizations also indicated that the element may be unique to canids and is absent in a variety of other mammals, including members of four closely-related carnivore families. Three examples of the SINE have been located and sequenced. The 130-bp SINE contains putative RNA polymerase III transcriptional control sequences. The SINE is flanked at the 3' end by a (TC)8-repeat region followed by a poly(A) tract of 35-65 nt. Computer database searches located two homologous sequences with approx. 80% identity to the SINE. These sequences were located in untranslated regions of the canine genes encoding interferon-omega and clotting factor IX.

摘要

从家犬（Canis familiaris）的基因组DNA中克隆出一种短散在核苷酸（nt）元件（SINE）。用四种限制性内切酶消化犬类DNA的Southern印迹分析表明，该SINE广泛分布于整个基因组中。杂交结果还表明，该元件可能是犬科动物所特有的，在包括四个亲缘关系密切的食肉动物家族成员在内的多种其他哺乳动物中不存在。已定位并测序了该SINE的三个实例。这个130bp的SINE包含推定的RNA聚合酶III转录控制序列。该SINE在3'端侧翼是一个(TC)8重复区域，后面跟着一个35 - 65 nt的聚腺苷酸序列。计算机数据库搜索找到了两个与该SINE具有约80%同源性的同源序列。这些序列位于编码干扰素ω和凝血因子IX的犬类基因的非翻译区。