家犬中一种丰富的短散在核元件（SINE）的特征分析。
Characterization of an abundant short interspersed nuclear element (SINE) present in Canis familiaris.
作者信息
Das M, Chu L L, Ghahremani M, Abrams-Ogg T, Roy M S, Housman D, Pelletier J
机构信息
Dept. of Biochemistry, McGill University, Montreal, Quebec, Canada.
出版信息
Mamm Genome. 1998 Jan;9(1):64-9. doi: 10.1007/s003359900681.
A short interspersed nuclear element (Can SINE) of approximately 130-150 bp was cloned and characterized from Canis familiaris. We demonstrate that this element is interspersed, present approximately every 5-8.3 kbp, and many are sufficiently close to allow IRS (interspersed repetitive DNA sequences) PCR. Sequence analysis of > 20 Can SINEs from the dog has identified a conserved region that was used to design oligonucleotides for IRS PCR. Since Can SINEs are not present in human or rodent genomes, IRS PCR using oligonucleotides directed to the conserved region of Can SINEs can be used to simplify analysis of canid DNA in somatic cell hybrids, as well as in large insert cloning vectors. We demonstrate that the canid IRS products are polymorphic and could be developed as genetic markers for filter-based genotyping in this organism.
从家犬中克隆并鉴定出一种约130 - 150 bp的短散在核元件（犬SINE）。我们证明该元件是散布的，大约每5 - 8.3 kbp出现一次，并且许多元件距离足够近，可用于IRS（散布重复DNA序列）PCR。对来自犬的20多个犬SINE进行序列分析，确定了一个保守区域，该区域用于设计IRS PCR的寡核苷酸。由于人类或啮齿动物基因组中不存在犬SINE，使用针对犬SINE保守区域的寡核苷酸进行IRS PCR可用于简化体细胞杂种以及大插入克隆载体中犬科动物DNA的分析。我们证明犬科动物的IRS产物具有多态性，可开发为该生物体基于滤膜基因分型的遗传标记。
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