Gasparac Rahela, Taft Bradford J, Lapierre-Devlin Melissa A, Lazareck Adam D, Xu Jimmy M, Kelley Shana O
Eugene F. Merkert Chemistry Center, Boston College, Chestnut Hill, Massachusetts 02467, USA.
J Am Chem Soc. 2004 Oct 6;126(39):12270-1. doi: 10.1021/ja0458221.
Electrochemical DNA detection systems are an attractive approach to the development of multiplexed, high-throughput DNA analysis systems for clinical and research applications. We have engineered a new class of nanoelectrode ensembles (NEEs) that constitute a useful platform for biomolecular electrochemical sensing. High-sensitivity DNA detection was achieved at oligonucleotide-functionalized NEEs using a label-free electrocatalytic assay. Attomole levels of DNA were detected using the NEEs, validating the promise of nanoarchitectures for ultrasensitive biosensing.
电化学DNA检测系统是开发用于临床和研究应用的多重、高通量DNA分析系统的一种有吸引力的方法。我们设计了一类新型的纳米电极阵列（NEEs），它们构成了用于生物分子电化学传感的有用平台。使用无标记电催化测定法在寡核苷酸功能化的NEEs上实现了高灵敏度DNA检测。使用NEEs检测到了阿托摩尔水平的DNA，验证了纳米结构用于超灵敏生物传感的前景。
