Tokiwa Takayoshi, Harada Kuniaki, Matsumura Toshiharu, Tukiyama Takashi
Kohno Clinical Medicine Research Institute, Shinagawa-ku, Tokyo, Japan.
Biol Pharm Bull. 2004 Oct;27(10):1691-3. doi: 10.1248/bpb.27.1691.
Periploca sepium (PS) has traditionally been used in oriental medicine for treatment of rheumatoid arthritis (RA). We investigated the aqueous extract of PS (PSE) in its effects on human rheumatoid arthritis-derived fibroblast-like cells. In cell culture studies, PSE inhibited the growth and IL-6 production of the cells in dose dependent manners. The extract of Glycyrrhiza glabra (GG), which has also been used to treat RA and chosen as a reference here, slightly inhibited the growth of RA cells. A study of PSE fractionation indicated that the active material inhibiting IL-6 production is filterable by ultrafiltration, suggesting that substances with low molecular weight might be involved in an inhibition of IL-6 production. These results support the view that PSE represents a rich source of growth inhibition and anti-IL 6 production.
杠柳（PS）在传统东方医学中一直用于治疗类风湿性关节炎（RA）。我们研究了杠柳水提取物（PSE）对人类风湿关节炎来源的成纤维样细胞的作用。在细胞培养研究中，PSE以剂量依赖的方式抑制细胞的生长和IL-6的产生。光果甘草（GG）提取物也被用于治疗RA，在此作为对照，它对RA细胞的生长有轻微抑制作用。对PSE分级分离的研究表明，抑制IL-6产生的活性物质可通过超滤过滤，这表明低分子量物质可能参与了对IL-6产生的抑制。这些结果支持了PSE是生长抑制和抗IL-6产生的丰富来源这一观点。
