Vervarcke Stefaan, Ollevier Frans, Kinget Renaat, Michoel Armand
Laboratorium voor Farmacotechnologie en Biofarmacie, Katholieke Universiteit Leuven, Campus Gasthuisberg O+N, Herestraat 49, 3000 Leuven, Belgium.
Fish Shellfish Immunol. 2005 Feb;18(2):125-33. doi: 10.1016/j.fsi.2004.06.004.
The aim of this study was to investigate the possibility of mucosal vaccination in African catfish (Clarias gariepinus) with Vibrio anguillarum O2 bacterins. The antigen was administered via different routes: anal intubation, oral administration, intraperitoneal injection and immersion. To monitor the antigen uptake, a competitive ELISA was used. The antibody response was measured using an indirect ELISA. Increased antibody levels were found in bile and mucus upon anal intubation, which was not the case after intraperitoneal injection. The data indicate that oral vaccination of fish may be possible when antigens can reach the second gut segment in sufficient quantities and in the right form as confirmed by the recorded substantial induction of systemic and mucosal immunity. The results obtained are a strong indication for mucosal immune response and the two compartmental models for immune response in fish.
本研究的目的是探讨用鳗弧菌O2菌苗对非洲鲶鱼（Clarias gariepinus）进行黏膜疫苗接种的可能性。抗原通过不同途径给药：肛门插管、口服、腹腔注射和浸泡。为监测抗原摄取情况，使用了竞争性ELISA。使用间接ELISA测量抗体反应。经肛门插管后，胆汁和黏液中的抗体水平升高，腹腔注射后则不然。数据表明，当抗原能够以足够的量并以正确的形式到达肠道第二部分时，鱼类口服疫苗接种可能是可行的，记录的全身和黏膜免疫的显著诱导证实了这一点。所获得的结果有力地表明了鱼类的黏膜免疫反应以及免疫反应的双区室模型。
