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层流手术室中细菌脱落的来源。

Source of bacterial shedding in laminar flow theatres.

作者信息

Owers K L, James E, Bannister G C

机构信息

Barnet and Chase Farm NHS Hospital Trust, UK.

出版信息

J Hosp Infect. 2004 Nov;58(3):230-2. doi: 10.1016/j.jhin.2004.06.028.

DOI:10.1016/j.jhin.2004.06.028
PMID:15501339
Abstract

In a previous unpublished observation, unacceptably high bacterial counts, presumably due to shedding episodes, occurred in two of 56 (3.57%) slit-air samples during arthroplasty surgery in a laminar flow operating theatre. The isolates matched those cultured from swab samples taken from the skin of one of the surgeons's foreheads on each of the two occasions. These findings occurred despite scrub staff wearing standard occlusive gowns, hats and masks with visors. In order to localize the potential source of such shedding more accurately, 20 members of theatre staff underwent anonymous microbiological swabbing from the facial areas not covered by theatre clothing, namely their foreheads, eyebrows and ears. These swabs were cultured and the growths were compared statistically. Significantly more colonies were cultured from swabs taken from the theatre staff's ears (P = 0.047, Freidman's test) compared with the other two facial areas studied. These data support the use of exhaust helmets in arthroplasty surgery, or at least mandatory coverage of the ears with theatre hats for scrub staff.

摘要

在之前一项未发表的观察中,在层流手术室进行关节置换手术期间,56份空气采样中的两份(3.57%)出现了高得不可接受的细菌计数,推测是由于脱落事件所致。分离出的细菌与两次手术时从其中一名外科医生前额皮肤拭子样本中培养出的细菌相匹配。尽管刷手人员穿着标准的封闭式手术衣、帽子和带面罩的口罩,仍出现了这些结果。为了更准确地确定这种脱落的潜在来源,20名手术室工作人员对未被手术服覆盖的面部区域,即前额、眉毛和耳朵进行了匿名微生物拭子采样。对这些拭子进行培养,并对培养结果进行统计学比较。与其他两个研究的面部区域相比,从手术室工作人员耳朵采集的拭子培养出的菌落明显更多(P = 0.047,弗里德曼检验)。这些数据支持在关节置换手术中使用排气头盔,或者至少要求刷手人员用手术帽强制覆盖耳朵。

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