Yang Xiao-Feng, Li Hua
Institute of Analytical Science, Northwest University, Xi'an 710069, China.
Talanta. 2004 Oct 8;64(2):478-83. doi: 10.1016/j.talanta.2004.03.013.
A novel flow-injection chemiluminescence (CL) method for the determination of dihydralazine sulfate (DHZS) is described. The method is based on the reaction between DHZS and hexacyanoferrate(III) in alkaline solution to give weak CL signal, which is dramatically enhanced by eosin Y. The CL emission allows quantitation of DHZS concentration in the range 0.02-2.8mugml(-1) with a detection limit (3sigma) of 0.012mugml(-1). The experimental conditions for the CL reaction are optimized and the possible reaction mechanism is discussed. The method has been applied to the determination of DHZS in pharmaceutical preparations and compared well with the high performance liquid chromatography (HPLC) method.
描述了一种用于测定硫酸肼屈嗪（DHZS）的新型流动注射化学发光（CL）方法。该方法基于DHZS与铁氰化钾（III）在碱性溶液中反应产生微弱的CL信号，而曙红Y可显著增强该信号。CL发射使得能够在0.02 - 2.8μg ml⁻¹范围内定量DHZS浓度，检测限（3σ）为0.012μg ml⁻¹。优化了CL反应的实验条件，并讨论了可能的反应机理。该方法已应用于药物制剂中DHZS的测定，与高效液相色谱（HPLC）法相比效果良好。
