Erdmann Thorsten, Schwarz Ulrich S
Max Planck Institute of Colloids and Interfaces, 14424 Potsdam, Germany.
J Chem Phys. 2004 Nov 8;121(18):8997-9017. doi: 10.1063/1.1805496.
Single receptor-ligand bonds have finite lifetimes, so that biological systems can dynamically react to changes in their environment. In cell adhesion, adhesion bonds usually act cooperatively in adhesion clusters. Outside the cellular context, adhesion clusters can be probed quantitatively by attaching receptors and ligands to opposing surfaces. Here we present a detailed theoretical analysis of the stochastic dynamics of a cluster of parallel bonds under shared constant loading and with rebinding. Analytical solutions for the appropriate one-step master equation are presented for special cases, while the general case is treated with exact stochastic simulations. If the completely dissociated state is modeled as an absorbing boundary, mean cluster lifetime is finite and can be calculated exactly. We also present a detailed analysis of fluctuation effects and discuss various approximations to the full stochastic description.
单个受体 - 配体键具有有限的寿命，因此生物系统能够对其环境变化做出动态反应。在细胞黏附中，黏附键通常在黏附簇中协同作用。在细胞环境之外，通过将受体和配体附着到相对表面上，可以对黏附簇进行定量探测。在此，我们对在共享恒定负载和存在重新结合情况下的平行键簇的随机动力学进行了详细的理论分析。针对特殊情况给出了适当的一步主方程的解析解，而一般情况则通过精确的随机模拟进行处理。如果将完全解离状态建模为吸收边界，则平均簇寿命是有限的并且可以精确计算。我们还对涨落效应进行了详细分析，并讨论了对完整随机描述的各种近似。
