Jötten Anna Martina, Moll Kirsten, Wahlgren Mats, Wixforth Achim, Westerhausen Christoph
Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Box 280, 171 77 Stockholm, Sweden.
Biomicrofluidics. 2020 Mar 20;14(2):024104. doi: 10.1063/1.5125038. eCollection 2020 Mar.
For related malaria (B50), one of the outstanding host factors for the development of severe disease is the ABO blood group of malaria patients, where blood group O reduces the probability of severe disease as compared to individuals of groups A, B, or AB. In this report, we investigate the stability of rosette aggregates in malaria caused by in microflows. These flows are created in microfluidic channels with stenosis-like constrictions of different widths down to ones narrower as the rosette's diameter. High speed videos were recorded and analyzed by a MATLAB© based tracking software (SURF: SUrvival of Rosettes in Flow). We find a correlation of rosette size, channel diameter, and blood group regarding the mobility of the rosettes. Following the concept of a thermodynamic model, we find a critical width of the stenosis for rosette rupture during their passage. Our data reveal that under physiologically relevant conditions, rosettes in blood group A have a higher rosette frequency and stability as compared to blood group O (BG O), which constitutes a crucial factor promoting the observed protection in BG O individuals against severe malaria in non-O individuals.
对于相关疟疾（B50），疟疾患者的ABO血型是严重疾病发生的一个突出宿主因素，与A、B或AB血型个体相比，O型血降低了患严重疾病的概率。在本报告中，我们研究了微流中疟疾引起的玫瑰花结聚集体的稳定性。这些流动是在微流体通道中产生的，通道具有不同宽度的类似狭窄的收缩部分，直至比玫瑰花结直径更窄的部分。通过基于MATLAB©的跟踪软件（SURF：流动中玫瑰花结的存活）记录并分析高速视频。我们发现玫瑰花结大小、通道直径和血型与玫瑰花结的流动性之间存在相关性。遵循热力学模型的概念，我们发现玫瑰花结在通过狭窄处时破裂的临界宽度。我们的数据表明，在生理相关条件下，与O型血（BG O）相比，A型血中的玫瑰花结具有更高的玫瑰花结频率和稳定性，这是促进观察到的BG O个体对非O型个体严重疟疾具有保护作用的关键因素。
