García-Moncó J C, Benach J L, Coleman J L, Galbe J L, Szczepanski A, Fernández Villar B, Norton Hughes C A, Johnson R C
Sección de Neurología, Hospital de Galdácano, Vizcaya.
Med Clin (Barc). 1992 Jan 25;98(3):89-93.
The characteristics of a Spanish strain of Borrelia burgdorferi, the spirochete which causes Lyme's disease, and which, up to the present, has not been isolated in Spain, are described.
The organism was obtained from ticks (Ixodes ricinus) from the northern part of Spain. It was studied in culture by dark field microscopy and the internal structure observed by electron transmission. The antigenic composition was analyzed under polyacrylamide electrophoresis, immunoblot and reactivity against monoclonal antibodies. Plasmid analysis was carried out by Southern blot.
In culture the length of the organism is somewhat shorter than normal. It grows slowly and tends to autoagglutinate. It has 6-13 periplasmic flagella. The antigenic analysis of this microorganism through immunoblot and reactivity against different monoclonal antibodies showed differences with regards to other North American strains, with the most significant being the composition of certain proteins of the surface of the organism. These differences may have clinical repercussion. DNA analysis by Southern blot demonstrated slight differences in regard to the composition of plasmids compared to other strains analyzed.
Borrelia burgdorferi exists in Spain. The isolated strain shows peculiar characteristics with respect to others analyzed. The availability of an autochthonous strain may allow more reliable serological diagnosis in Spain.
描述了一种西班牙株伯氏疏螺旋体的特征，该螺旋体可引发莱姆病，且迄今为止尚未在西班牙分离得到。
该生物体取自西班牙北部的蜱（蓖麻硬蜱）。通过暗视野显微镜在培养物中对其进行研究，并通过电子透射观察其内部结构。在聚丙烯酰胺电泳、免疫印迹以及与单克隆抗体的反应性分析下对抗抗原组成进行分析。通过Southern印迹进行质粒分析。
在培养物中，该生物体的长度略短于正常长度。其生长缓慢且倾向于自身凝集。它有6 - 13根周质鞭毛。通过免疫印迹以及与不同单克隆抗体的反应性对这种微生物进行的抗原分析显示，与其他北美菌株存在差异，最显著的是该生物体表面某些蛋白质的组成。这些差异可能具有临床影响。通过Southern印迹进行的DNA分析表明，与其他分析的菌株相比，质粒组成存在细微差异。
伯氏疏螺旋体存在于西班牙。分离出的菌株与其他分析的菌株相比表现出独特的特征。本地菌株的可得性可能使西班牙的血清学诊断更加可靠。
