Ding Linxian, Yokota Akira
Laboratory of Bioresources, Institute of Molecular and Cellular Biosciences, The University of Tokyo, Yayoi, Bunkyo-ku, Tokyo 113-0032, Japan.
Int J Syst Evol Microbiol. 2004 Nov;54(Pt 6):2223-2230. doi: 10.1099/ijs.0.02975-0.
Two strains of curved bacteria, 7-1(T) and 7-2(T), isolated from well water, were phylogenetically examined to determine their taxonomic position. Strain 7-1(T) is a Gram-negative, slightly curved rod. Analysis of the 16S rRNA gene sequence showed that strain 7-1(T) formed a cluster with [Aquaspirillum] delicatum and [Pseudomonas] lanceolata. It has some similar characteristics to [A.] delicatum and [P.] lanceolata, but has sufficient distance to separate it from other genera. DNA-DNA hybridization analysis, as well as chemotaxonomic and morphological studies, demonstrated that strain 7-1(T), [A.] delicatum and [P.] lanceolata belong to a new genus, Curvibacter gen. nov. Strain 7-1(T) (=IAM 15033(T)=ATCC BAA-807(T)) is classified as the type strain of Curvibacter gracilis gen. nov., sp. nov., and [A.] delicatum and [P.] lanceolata are classified as Curvibacter delicatus comb. nov. and Curvibacter lanceolatus comb. nov., respectively. Strain 7-2(T) is a Gram-negative spirillum. Phylogenetic study based on the 16S rRNA gene sequences showed that it formed a cluster with the members of the genus Herbaspirillum, [Pseudomonas] huttiensis and [Aquaspirillum] autotrophicum. The classification is therefore proposed of strain 7-2(T) (=IAM 15032(T)=ATCC BAA-806(T)) as the type strain of Herbaspirillum putei sp. nov., and [P.] huttiensis and [A.] autotrophicum are transferred to the genus Herbaspirillum as Herbaspirillum huttiense comb. nov. and Herbaspirillum autotrophicum comb. nov., respectively.
从井水分离出的两株弯曲菌，7-1(T)和7-2(T)，进行了系统发育分析以确定它们的分类地位。菌株7-1(T)是革兰氏阴性、稍弯曲的杆菌。16S rRNA基因序列分析表明，菌株7-1(T)与纤细水生螺菌（Aquaspirillum delicatum）和披针假单胞菌（Pseudomonas lanceolata）形成一个簇。它与纤细水生螺菌和披针假单胞菌有一些相似特征，但有足够的差异将其与其他属区分开来。DNA-DNA杂交分析以及化学分类和形态学研究表明，菌株7-1(T)、纤细水生螺菌和披针假单胞菌属于一个新属，弯曲杆菌属（Curvibacter gen. nov.）。菌株7-1(T)（=IAM 15033(T)=ATCC BAA-807(T)）被分类为纤细弯曲杆菌（Curvibacter gracilis gen. nov., sp. nov.）的模式菌株，纤细水生螺菌和披针假单胞菌分别被分类为纤细弯曲杆菌（Curvibacter delicatus comb. nov.）和披针弯曲杆菌（Curvibacter lanceolatus comb. nov.）。菌株7-2(T)是革兰氏阴性螺菌。基于16S rRNA基因序列的系统发育研究表明，它与草螺菌属（Herbaspirillum）、胡氏假单胞菌（Pseudomonas huttiensis）和自养水生螺菌（Aquaspirillum autotrophicum）的成员形成一个簇。因此，建议将菌株7-2(T)（=IAM 15032(T)=ATCC BAA-806(T)）分类为井水草螺菌（Herbaspirillum putei sp. nov.）的模式菌株，胡氏假单胞菌和自养水生螺菌分别作为胡氏草螺菌（Herbaspirillum huttiense comb. nov.）和自养草螺菌（Herbaspirillum autotrophicum comb. nov.）转移到草螺菌属。
