Kaygusuz Irfan, Gödekmerdan Ahmet, Keleş Erol, Karlidağ Turgut, Yalçin Sinasi, Yildiz Mücahit, Tazegül Ayça
Department of Otorhinolaryngology, Firat University Medical Center, 23200 Elaziğ, Turkey.
Am J Otolaryngol. 2004 Nov-Dec;25(6):401-6. doi: 10.1016/j.amjoto.2004.04.011.
The purpose of this study is to investigate the role of viruses on the idiopathic peripheral facial palsy and show the interaction of immune system.
The levels of immunoglobulin (Ig) G and IgM antibodies against to varicella-zoster virus (VZV), herpes simplex virus (HSV), cytomegalovirus (CMV), Ebstein-Barr virus (EBV), and mumps virus in venous blood taken from patients in the amount of 10 mL have been investigated by enzyme-linked immunosorbent assay method. Tumor necrosis factor-alpha (TNF-alpha), interferon-gamma (IFN-gamma), and transforming growth factor-beta (TGF-beta) were also examined. Of lymphocyte subpopulation, antibodies of CD3(+), CD4(+), CD8(+), CD19(+), and CD16(+) plus 56(+) were analyzed.
Ten of the patients had HSV-1 IgG; 1 of the patients IgM, 5 of the patients EBV IgG, 6 of the patients VZV IgG, 1 of the patients IgM, 9 of the patients mumps IgG, 1 of the patients IgM, and finally in 7 of the patients CMV IgG antibodies were obtained. It was found that CD4(+) cell and ratio of CD4(+)/CD8(+) lower and the percentage of the CD8(+) and CD16(+) plus 56(+) cells higher compared with the control group (P < .05). The levels of TNF-alpha were lower, whereas IFN-gamma and TGF-beta1 were higher.
It may be concluded from these results that VZV, HSV-1, CMV, EBV, and mumps virus play a significant role in the etiology of idiopathic peripheral facial palsy and activate the cellular immunity.
本研究旨在探讨病毒在特发性周围性面神经麻痹中的作用，并展示免疫系统的相互作用。
采用酶联免疫吸附测定法，对从10毫升静脉血中采集的患者血清中针对水痘-带状疱疹病毒（VZV）、单纯疱疹病毒（HSV）、巨细胞病毒（CMV）、EB病毒（EBV）和腮腺炎病毒的免疫球蛋白（Ig）G和IgM抗体水平进行了检测。同时检测了肿瘤坏死因子-α（TNF-α）、干扰素-γ（IFN-γ）和转化生长因子-β（TGF-β）。对淋巴细胞亚群中的CD3(+)、CD4(+)、CD8(+)、CD19(+)以及CD16(+)加56(+)抗体进行了分析。
10例患者检测到HSV-1 IgG；1例患者检测到IgM，5例患者检测到EBV IgG，6例患者检测到VZV IgG，1例患者检测到IgM，9例患者检测到腮腺炎IgG，1例患者检测到IgM，最后7例患者检测到CMV IgG抗体。结果发现，与对照组相比，患者的CD4(+)细胞及CD4(+)/CD8(+)比值降低，CD8(+)和CD16(+)加56(+)细胞百分比升高（P <.05）。TNF-α水平较低，而IFN-γ和TGF-β1水平较高。
从这些结果可以得出结论，VZV、HSV-1、CMV、EBV和腮腺炎病毒在特发性周围性面神经麻痹的病因中起重要作用，并激活细胞免疫。
