Department of Pathology, Faculty of Veterinary Medicine, University of Wijaya Kusuma Surabaya, Surabaya, Indonesia.
Department of Basic Veterinary Medicine, Faculty of Veterinary, Universitas Airlangga, Kampus C UNAIR, Surabaya, Indonesia.
Open Vet J. 2024 Sep;14(9):2250-2255. doi: 10.5455/OVJ.2024.v14.i9.12. Epub 2024 Sep 30.
Diabetes mellitus is a significant global health issue with increasing prevalence
This study aims to investigate the potential of extract (TE) in lowering interleukin-35 (IL-35), tumor necrosis factor-alpha (TNF-α), and hematological profile in streptozotocin (STZ)-induced rats.
A total of 24 rats were divided into four treatment groups: control (P0), diabetic induction (P1), diabetic induction + TE (P2), and diabetic induction + quercetin (P3). Diabetes mellitus was induced by a single-dose injection of stz (60 mg/kg). TE treatment was administered orally for 7 days. On the 8th day post-treatment, all animals were euthanized, and blood samples were collected to assess inflammatory parameters, including IL-35, TNF-α, GPx, and hematological profiles. Kidney organs were fixed in 10% buffered neutral formalin for histopathological analysis. Data were analyzed using ANOVA followed by Duncan's test ( < 0.05).
Evaluation of the hematological profile revealed significant improvements in the P2 and P3 groups, with decreased leukocytes, hemoglobin, lymphocytes, and neutrophils, as well as significantly lower IL-35 and TNF-α levels observed in diabetic rats following TE treatment.
TE treatment exhibited promising effects in reducing inflammatory markers and restoring hematological parameters in diabetic rats, indicating its potential as a therapeutic agent in diabetic rats.
糖尿病是一个全球性的重大健康问题，其患病率正在不断上升。
本研究旨在探讨 提取物（TE）降低链脲佐菌素（STZ）诱导的大鼠白细胞介素 35（IL-35）、肿瘤坏死因子-α（TNF-α）和血液学特征的潜力。
将 24 只大鼠分为四组：对照组（P0）、糖尿病诱导组（P1）、糖尿病诱导+TE 组（P2）和糖尿病诱导+槲皮素组（P3）。通过单次注射 STZ（60mg/kg）诱导糖尿病。TE 治疗通过口服给药进行 7 天。在治疗后第 8 天，所有动物被安乐死，采集血液样本以评估炎症参数，包括 IL-35、TNF-α、GPx 和血液学特征。肾脏器官用 10%缓冲中性福尔马林固定进行组织病理学分析。使用方差分析（ANOVA）和 Duncan 检验（<0.05）进行数据分析。
血液学特征评估显示，P2 和 P3 组有显著改善，白细胞、血红蛋白、淋巴细胞和中性粒细胞减少，糖尿病大鼠经 TE 治疗后 IL-35 和 TNF-α 水平显著降低。
TE 治疗在降低糖尿病大鼠的炎症标志物和恢复血液学参数方面表现出有希望的效果，表明其可能成为糖尿病大鼠的治疗剂。
