Levis Silvana, Garcia Jorge, Pini Noemí, Calderón Gladys, Ramírez Josefina, Bravo Daniel, St Jeor Stephen, Ripoll Carlos, Bego Mariana, Lozano Elena, Barquez Rubén, Ksiazek Thomas G, Enria Delia
Instituto Nacional de Enfermedades Virales Humanas Dr. Julio I. Maiztegui, Monteagudo 2510, 2700 Pergamino, Buenos Aires, Argentina.
Am J Trop Med Hyg. 2004 Nov;71(5):658-63.
The purpose of this study was to characterize the hantaviruses circulating in northwestern Argentina. Human and rodent studies were conducted in Yuto, where most cases of hantavirus pulmonary syndrome (HPS) occur. Partial virus genome sequences were obtained from the blood of 12 cases of HPS, and from the lungs of 4 Calomys callosus and 1 Akodon simulator. Phylogenetic analysis showed that three genotypes associated with HPS circulate in Yuto. Laguna Negra (LN) virus, associated with C. laucha in Paraguay, was identified for the first time in Argentina; it was recovered from human cases and from C. callosus samples. The high sequence identity between human and rodent samples implicated C. callosus as the primary rodent reservoir for LN virus in Yuto. The genetic analysis showed that the Argentinian LN virus variant differed 16.8% at the nucleotide level and 2.9% at the protein level relative to the Paraguayan LN virus. The other two hantavirus lineages identified were the previously known Bermejo and Oran viruses.
本研究的目的是对阿根廷西北部流行的汉坦病毒进行特征描述。在尤托开展了人类和啮齿动物研究，汉坦病毒肺综合征（HPS）的大多数病例都发生在该地。从12例HPS患者的血液以及4只罗氏白足鼠和1只辛氏稻鼠的肺中获取了部分病毒基因组序列。系统发育分析表明，与HPS相关的三种基因型在尤托流行。首次在阿根廷发现了与巴拉圭罗氏白足鼠相关的拉古纳内格拉（LN）病毒；该病毒从人类病例和罗氏白足鼠样本中分离得到。人类和啮齿动物样本之间的高序列同一性表明罗氏白足鼠是尤托LN病毒的主要啮齿动物宿主。遗传分析表明，阿根廷LN病毒变种与巴拉圭LN病毒相比，在核苷酸水平上有16.8%的差异，在蛋白质水平上有2.9%的差异。鉴定出的另外两个汉坦病毒谱系是先前已知的贝梅霍病毒和奥兰病毒。
