Cvetnić Zdenka, Vladimir-Knezević Sanda
Department of Microbiology, Faculty of Pharmacy and Biochemistry, University of Zagreb, Croatia.
Acta Pharm. 2004 Sep;54(3):243-50.
Antibacterial and antifungal activity of ethanolic extract of grapefruit (Citrus paradisi Macf., Rutaceae) seed and pulp was examined against 20 bacterial and 10 yeast strains. The level of antimicrobial effects was established using an in vitro agar assay and standard broth dilution susceptibility test. The contents of 3.92% of total polyphenols and 0.11% of flavonoids were determined spectrometrically in crude ethanolic extract. The presence of flavanones naringin and hesperidin in the extract was confirmed by TLC analysis. Ethanolic extract exibited the strongest antimicrobial effect against Salmonella enteritidis (MIC 2.06%, m/V). Other tested bacteria and yeasts were sensitive to extract concentrations ranging from 4.13% to 16.50% (m/V).
对葡萄柚（柑橘属，芸香科）种子和果肉的乙醇提取物针对20种细菌菌株和10种酵母菌株进行了抗菌和抗真菌活性检测。使用体外琼脂试验和标准肉汤稀释药敏试验确定抗菌效果水平。通过光谱法测定粗乙醇提取物中总多酚含量为3.92%，黄酮类化合物含量为0.11%。通过薄层色谱分析确认提取物中存在黄烷酮柚皮苷和橙皮苷。乙醇提取物对肠炎沙门氏菌表现出最强的抗菌作用（最低抑菌浓度为2.06%，质量/体积）。其他受试细菌和酵母对浓度范围为4.13%至16.50%（质量/体积）的提取物敏感。
