Koregol Arati C, Kalburgi Nagaraj B, Puttarevanna Tejashwini, Patil Ruchita S, Singh Prerna, Sulakod Kavya
Department of Periodontics, P.M.N.M. Dental College and Hospital, Rajiv Gandhi University of Health Sciences. India.
Med Pharm Rep. 2022 Apr;95(2):185-190. doi: 10.15386/mpr-1978. Epub 2022 Apr 28.
Grape seed extract contains Proanthocyanin, which reduces collagen degradation by inhibiting interstitial and extracellular collagenase, thus having the potential to reduce the progression of periodontitis. Here we compare and evaluate the aerobic and anaerobic microorganism's CFUs (colony forming units) in plaque samples of Group A, Group B, Group C and severity of periodontal disease on day 0 and 7.
Forty-five subjects in age range 18-30 years were selected among undergraduate students and randomly divided into Group A: 15, 2% grape seed extract mouthwash (GSE), Group B: 15, 0.2% chlorhexidine mouthwash (CHX) and Group C: 15, distilled water (control). The supragingival plaque was collected into transport media. Kruskal Wallis test followed by Mann Whitney test was used to compare the mean CFUs (×10) of microorganisms and severity of periodontal disease was compared, by clinical parameters among all groups on day 0 and 7.
There was a significant difference concerning mean scores of all clinical parameters (P<0.001) and mean CFUs of microorganisms between 3 study groups (P=0.005) at 7 days post-intervention period. Intragroup comparison, mean scores were significantly reduced on day 7 as compared to day 0 at (P<0.001) in Group A and B, but no significant difference was noted with Group C.
Intervention with GSE mouthwash showed a positive effect on reducing CFUs in the plaque when compared with the control group. GSE group also showed similar results in reducing CFUs in plaque when compared to CHX group, thereby demonstrating the agent's antimicrobial efficacy, therapeutic effect and its potential usefulness in controlling plaque and periodontal diseases.
葡萄籽提取物含有原花青素，它通过抑制间质和细胞外胶原酶来减少胶原蛋白降解，因此有可能减缓牙周炎的进展。在此，我们比较并评估A组、B组、C组菌斑样本中需氧和厌氧微生物的菌落形成单位（CFU）以及第0天和第7天牙周疾病的严重程度。
从本科生中选取45名年龄在18至30岁之间的受试者，随机分为A组：15人，使用2%葡萄籽提取物漱口水（GSE）；B组：15人，使用0.2%氯己定漱口水（CHX）；C组：15人，使用蒸馏水（对照组）。将龈上菌斑收集到转运培养基中。采用Kruskal Wallis检验，随后进行Mann Whitney检验，以比较微生物的平均CFU（×10），并通过临床参数比较所有组在第0天和第7天牙周疾病的严重程度。
干预7天后，3个研究组在所有临床参数的平均得分（P<0.001）和微生物的平均CFU方面存在显著差异（P=0.005）。组内比较，A组和B组在第7天的平均得分与第0天相比显著降低（P<0.001），但C组未观察到显著差异。
与对照组相比，使用GSE漱口水进行干预对减少菌斑中的CFU有积极作用。与CHX组相比，GSE组在减少菌斑中的CFU方面也显示出相似的结果，从而证明了该制剂的抗菌功效、治疗效果及其在控制菌斑和牙周疾病方面的潜在用途。
