巨细胞病毒pp-65抗原血症检测与血浆DNA与移植受者临床结局的相关性比较

Comparison of cytomegalovirus pp-65 antigenemia assay and plasma DNA correlation with the clinical outcome in transplant recipients.

作者信息

de Oña María, Melón Santiago, Galarraga María C, Palacio Ana, Lambert José L, Bernardo María J, Rodríguez Manuel, Gómez Ernesto

机构信息

Services of Microbiology, HUCA, 33006 Oviedo, Asturias, Spain.

出版信息

Transpl Int. 2005 Jan;18(1):43-6. doi: 10.1111/j.1432-2277.2004.00033.x.

DOI:10.1111/j.1432-2277.2004.00033.x

PMID:15612982

Abstract

The relationship between quantitative antigenemia and plasma DNAemia was studied for monitoring cytomegalovirus (CMV) viremia in CMV infection (CMVI) or disease (CMVD), in 20 transplant recipients (13 CMD, seven CMVI). A total of 142 samples of blood were assayed for CMV-DNA and pp-65 antigenemia (CMV-Ag). A quantitative correlation between both markers was found (P < 0.0001). First CMV antigenemia as well as first plasma DNA viral load was similar in CMVI and CMVD (29 vs. 24 CMV-Ag+ cells/10(5) PMN; and 7445 vs. 12407 CMV-DNA copies/ml). The maximum antigenemia was higher in CMVD than in CMVI (146 +/- 87 vs. 61 +/- 54 +cells/10(5) PMN, P < 0.05), but the highest CMV plasma viral load was similar in both groups (62592 +/- 33000 vs. 42055 +/- 38600 copies/ml). In nine patients, maximum antigenemia coincided with highest plasma DNA viral load, but in 10 highest DNAemia occurred 6 days later. On the contrary, antigenemia declined faster than CMV-DNAnemia, after treatment.

摘要

为监测巨细胞病毒（CMV）感染（CMVI）或疾病（CMVD）中的CMV病毒血症，研究了20例移植受者（13例CMVD，7例CMVI）的定量抗原血症与血浆DNA血症之间的关系。共对142份血液样本检测了CMV-DNA和pp-65抗原血症（CMV-Ag）。发现两种标志物之间存在定量相关性（P<0.0001）。CMVI和CMVD中的首次CMV抗原血症以及首次血浆DNA病毒载量相似（分别为29对24个CMV-Ag+细胞/10⁵个PMN；以及7445对12407个CMV-DNA拷贝/ml）。CMVD中的最大抗原血症高于CMVI（146±87对61±54个细胞/10⁵个PMN，P<0.05），但两组中的最高CMV血浆病毒载量相似（分别为62592±33000对42055±38600拷贝/ml）。在9例患者中，最大抗原血症与最高血浆DNA病毒载量同时出现，但在10例患者中，最高DNA血症在6天后出现。相反，治疗后抗原血症的下降速度比CMV-DNAnemia更快。