Tang Hanjun, Inoue Mamiko, Uzawa Yuki, Kawamura Yukio
Department of Applied Life Chemistry, Graduate School of Agriculture, Kinki University, Nara 631-0052, Japan.
Biofactors. 2004;22(1-4):107-10. doi: 10.1002/biof.5520220121.
Anti-tumorigenic action of compounds in some seaweed was evaluated by using selective cytotoxicity against a transformed mouse 3T3 cell (SV-T2) and its normal counter part. The extracts of seaweeds (Wakame, Konbu, Mirin, Aosa, and Sujiaonori) were added to the cells in 96 well microplate. The viability of the cell was measured at 24 hr after sample addition. Of the five weeds, the ethanol extracts from Sujiaonori exhibited the selective cytotoxicity. The ethanol extracts from Sujiaonori was applied to gel permeation chromatography on Sephadex G-50. The anti-cancer activity was detected in two fractions with high molecular weight near void volume and with small molecular weight. The high molecular weight fractions had absorbance at 280 nm, suggesting that they contained proteineous substances. The small molecular weight fractions were estimated to be around 500 Dalton.
通过对转化的小鼠3T3细胞（SV-T2）及其正常对照细胞的选择性细胞毒性，评估了某些海藻中化合物的抗肿瘤作用。将海藻（裙带菜、海带、甜料酒、绿藻和条斑紫菜）提取物添加到96孔微孔板中的细胞中。在添加样品24小时后测量细胞活力。在这五种海藻中，条斑紫菜的乙醇提取物表现出选择性细胞毒性。将条斑紫菜的乙醇提取物应用于Sephadex G-50凝胶渗透色谱。在接近空体积的高分子量和低分子量的两个组分中检测到抗癌活性。高分子量组分在280nm处有吸光度，表明它们含有蛋白质类物质。低分子量组分估计约为500道尔顿。
