Duran I, Sengun A, Yildirim T, Ozturk B
Selcuk University, School of Dentistry, 42079 Konya, Turkey.
J Oral Rehabil. 2005 Jan;32(1):34-8. doi: 10.1111/j.1365-2842.2004.01132.x.
The aim of this study was to evaluate in vitro dentine permeability evaluation of 2-Hydroxyethyl methacrylate (HEMA)-based desensitizing products using split-chamber model following in vivo application in the dogs for three experimental time periods of 1 week, 1 month and 3 months. Buccal enamel of upper and lower canines of nine young dogs was removed and flat dentinal surface was obtained using a water-cooled diamond bur. The dentinal surface divided into four quarters, three experimental and a control. Health-Dent and Gluma Desensitizing agent and Single bond were applied to respective quarters of one tooth, according to manufacturers' instructions. The last quarters were left as control without any applications. For the each experimental period, 10 dentine samples from each group including control were used to measure the hydraulic conductance values. The dentine discs were placed pulp-side down in a split-chamber device in which the plastic spacers containing the rubber 0 rings have a surface area of 1 mm(2) and permeability was measured by fluid filtration. The data were expressed as hydraulic conductance (Lp). Differences in dentine permeability obtained for the desensitizing agents against the control were tested for statistical significance using Kruskal-Wallis one-way ANOVA and Bonferroni-adjusted Mann-Whitney U-tests. Differences in permeability in three time periods for each desensitizing agent were analysed using Friedman's anova and Wilcoxon signed rank tests. All desensitizing applications caused decreased Lp values compared with the control Lp value at the end of 1 week. In the 1-month samples, between the Lp values of Single bond and control, no statistical difference was found (P > 0.05). At the end of the 3-month period, the Gluma Desensitizing agent had the lowest the Lp value (P < 0.05). The result of this study is that the topical application of desensitizing agents led to decrease in dentine permeability in the dog model. The Gluma Desensitizing agent provide the more lasting tubule-occluding effect than the other material tested in this model.
本研究的目的是在犬体内应用2-羟乙基甲基丙烯酸酯（HEMA）基脱敏产品1周、1个月和3个月这三个实验时间段后，使用分室模型评估其体外牙本质渗透性。选取9只幼犬的上下颌犬齿，去除颊侧牙釉质，使用水冷金刚石车针获得平整的牙本质表面。将牙本质表面分为四个区域，三个实验区和一个对照区。按照制造商的说明，将Health-Dent、Gluma脱敏剂和Single bond分别应用于一颗牙齿的相应区域。最后一个区域留作对照，不做任何处理。在每个实验时间段，从每组（包括对照组）中选取10个牙本质样本测量水力传导值。将牙本质圆盘牙髓侧朝下放置在分室装置中，其中含有橡胶0形环的塑料垫片表面积为1平方毫米，通过流体过滤测量渗透性。数据以水力传导率（Lp）表示。使用Kruskal-Wallis单因素方差分析和Bonferroni校正的Mann-Whitney U检验对脱敏剂与对照相比牙本质渗透性的差异进行统计学显著性检验。使用Friedman方差分析和Wilcoxon符号秩检验分析每种脱敏剂在三个时间段内渗透性的差异。与1周结束时的对照Lp值相比，所有脱敏处理均导致Lp值降低。在1个月的样本中，Single bond和对照的Lp值之间未发现统计学差异（P>0.05）。在3个月结束时，Gluma脱敏剂的Lp值最低（P<0.05）。本研究的结果是，在犬模型中，局部应用脱敏剂可导致牙本质渗透性降低。在该模型中，Gluma脱敏剂比其他测试材料提供更持久的小管封闭效果。
