Dunn B E, Roop R M, Sung C C, Sharma S A, Perez-Perez G I, Blaser M J
Laboratory Service, John L. McClellan Memorial Veterans Hospital, Little Rock, Arkansas.
Infect Immun. 1992 May;60(5):1946-51. doi: 10.1128/iai.60.5.1946-1951.1992.
Helicobacter pylori is associated with gastritis and peptic ulcer disease in humans. We have identified a homolog of the chaperonin cpn60 family of heat shock proteins in H. pylori, referred to as Hp54K. Hp54K, purified from water-extractable H. pylori proteins, migrated as a single band at 54 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Its native molecular mass was 740 kDa; thus, Hp54K apparently comprises a 14-mer. The N-terminal 33 residues of Hp54K exhibited 60.6, 57.6, 54.5, 54.5, 51.5, and 51.5% identity with corresponding sequences in the following cpn60 homologs: HtpB (Legionella pneumophila), P1 (human mitochondria), GroEL (Escherichia coli), BA60K (Brucella abortus), HypB (Chlamydia trachomatis), and the 65-kDa immunodominant protein of Mycobacterium bovis BCG, respectively. Hp54K was the only protein recognized in whole-cell preparations of H. pylori by immunoblotting using monospecific antisera against cpn60 homologs from L. pneumophila, E. coli, C. trachomatis, and M. bovis BCG. Antiserum against Hp54K recognized proteins with molecular masses of 50 to 60 kDa in a large number of gram-negative bacteria, consistent with the known highly conserved nature of cpn60 proteins. Hp54K is a major protein and is immunogenic in humans infected with H. pylori. Thus, Hp54K shares many similarities with known cpn60 homologs. On the basis of the proposed role of other cpn60 proteins in induction of chronic inflammation, immune cross-reactivity between Hp54K and gastric tissue may provide an important link between H. pylori infection and gastritis.
幽门螺杆菌与人类的胃炎和消化性溃疡疾病相关。我们在幽门螺杆菌中鉴定出一种热休克蛋白伴侣蛋白cpn60家族的同源物，称为Hp54K。从可水提取的幽门螺杆菌蛋白中纯化得到的Hp54K，通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳在54 kDa处迁移为单一条带。其天然分子质量为740 kDa；因此，Hp54K显然由一个14聚体组成。Hp54K的N端33个残基与以下cpn60同源物的相应序列分别具有60.6%、57.6%、54.5%、54.5%、51.5%和51.5%的同一性：嗜肺军团菌的HtpB、人线粒体的P1、大肠杆菌的GroEL、流产布鲁氏菌的BA60K、沙眼衣原体的HypB以及牛分枝杆菌卡介苗的65 kDa免疫显性蛋白。使用针对嗜肺军团菌、大肠杆菌、沙眼衣原体和牛分枝杆菌卡介苗的cpn60同源物的单特异性抗血清进行免疫印迹分析时，Hp54K是幽门螺杆菌全细胞制剂中唯一被识别的蛋白。针对Hp54K的抗血清在大量革兰氏阴性细菌中识别出分子量为50至60 kDa的蛋白，这与已知的cpn60蛋白高度保守的性质一致。Hp54K是一种主要蛋白，在感染幽门螺杆菌的人类中具有免疫原性。因此，Hp54K与已知的cpn60同源物有许多相似之处。基于其他cpn60蛋白在诱导慢性炎症中的假定作用，Hp54K与胃组织之间的免疫交叉反应性可能为幽门螺杆菌感染与胃炎之间提供重要联系。
