Pérez-Pérez G I, Brown W R, Cover T L, Dunn B E, Cao P, Blaser M J
Department of Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA.
Clin Diagn Lab Immunol. 1994 May;1(3):325-9. doi: 10.1128/cdli.1.3.325-329.1994.
In 82 patients who underwent gastroduodenoscopy, acute and chronic gastric mucosal inflammation was scored for severity, and systemic humoral immune responses to Helicobacter pylori antigens were assessed by enzyme-linked immunosorbent assays. On the basis of culture, gastric histology, and serologic evaluation, 33 patients were classified as H. pylori infected and 36 were classified as uninfected. Thirteen patients had negative cultures and stains but were seropositive and were analyzed separately from the other two groups. Specific serum immunoglobulin G (IgG) subclass responses to H. pylori whole-cell antigens and specific IgG responses to the 54-kDa heat shock protein homolog (Hp54K) and vacuolating cytotoxin were significantly greater in infected than in uninfected patients as were specific IgA responses to whole-cell antigens and cytotoxin (P < 0.001). Among the H. pylori-infected persons, serum IgG responses to Hp54K and to the vacuolating cytotoxin were correlated with acute mucosal inflammatory scores. In contrast, serum IgA responses to whole-cell sonicate and to vacuolating cytotoxin were inversely related to chronic inflammatory scores. By multivariant regression analysis, only specific serum IgG responses to Hp54K correlated with severity of inflammation (both acute and chronic; P < 0.001); these responses may be markers of inflammation or these antibodies could play a direct role in the pathogenesis of H. pylori-induced inflammation.
在82例行胃十二指肠镜检查的患者中，对急性和慢性胃黏膜炎症的严重程度进行评分，并通过酶联免疫吸附测定评估对幽门螺杆菌抗原的全身体液免疫反应。根据培养、胃组织学和血清学评估，33例患者被分类为幽门螺杆菌感染，36例被分类为未感染。13例患者培养和染色均为阴性，但血清学呈阳性，与其他两组分开分析。感染患者对幽门螺杆菌全细胞抗原的特异性血清免疫球蛋白G（IgG）亚类反应、对54 kDa热休克蛋白同源物（Hp54K）和空泡毒素的特异性IgG反应，以及对全细胞抗原和毒素的特异性IgA反应均显著高于未感染患者（P < 0.001）。在幽门螺杆菌感染患者中，血清对Hp54K和空泡毒素的IgG反应与急性黏膜炎症评分相关。相反，血清对全细胞超声裂解物和空泡毒素的IgA反应与慢性炎症评分呈负相关。通过多变量回归分析，只有血清对Hp54K的特异性IgG反应与炎症严重程度相关（急性和慢性均相关；P < 0.001）；这些反应可能是炎症的标志物，或者这些抗体可能在幽门螺杆菌诱导的炎症发病机制中起直接作用。
