Liew C, Curtis C F
Environmental Health Institute, National Environment Agency, Singapore.
Med Vet Entomol. 2004 Dec;18(4):351-60. doi: 10.1111/j.0269-283X.2004.00517.x.
To study the dispersal of dengue vector mosquitoes in Singapore, females of Aedes aegypti (L.) and Aedes albopictus (Skuse) (Diptera: Culicidae) were fed blood containing rubidium (Rb), which was detectable in their eggs by means of Graphite Furnace Atomic Absorption Spectrophotometry (GFAAS). Laboratory calibration of the Rb reading, for a range of egg numbers from Rb-fed females, indicated a reasonably linear relationship and an unequivocal distinction between results with zero and one marked egg. Rb-marked female Aedes mosquitoes aged 3-5 days were released in semi-rural and urbanized parts of Singapore, with an array of ovitraps extending to a radius of 320 m from the release point. Subsequently, Rb-marked Aedes eggs were detected throughout the array, with similar distributions on each of the 4 days after release. More Rb was detected nearer the release point. However, when correction was made for the greater areas of zones further from the release point (and therefore presumably existence of more alternative oviposition sites), there were no significant differences in the numbers of marked eggs per ovitrap in the zones nearer or further from the release points. It is concluded that females of both these Aedes (Stegomyia) species could disperse easily and quickly throughout areas of radius 320 m in search of oviposition sites. This contrasts with the general belief that Ae. aegypti seldom flies more than 50 m and that control operations can safely be based on such an assumption. Releases on level 12 of a 21-storey apartment block, with ovitraps on each storey, showed similar easy and rapid dispersal to the top and bottom of the block.
为研究登革热媒介蚊虫在新加坡的扩散情况，埃及伊蚊（L.）和白纹伊蚊（Skuse）（双翅目：蚊科）的雌蚊被喂食含铷（Rb）的血液，通过石墨炉原子吸收分光光度法（GFAAS）可在其产下的卵中检测到铷。对喂食铷的雌蚊所产一系列数量卵的铷读数进行实验室校准，结果表明两者存在合理的线性关系，且零枚标记卵和一枚标记卵的结果有明确区分。将3至5日龄的铷标记埃及伊蚊雌蚊释放于新加坡的半乡村和城市化地区，一系列诱蚊产卵器分布在距释放点半径达320米的范围内。随后，在整个分布区域均检测到铷标记的伊蚊卵，释放后4天里每天的分布情况相似。在距释放点较近的区域检测到更多的铷。然而，对距释放点较远区域（因此可能存在更多替代产卵场所）的更大面积进行校正后，距释放点较近和较远区域每个诱蚊产卵器中标记卵的数量并无显著差异。得出结论，这两种伊蚊（覆蚊亚属）雌蚊能够轻松快速地在半径320米的区域内扩散以寻找产卵场所。这与普遍认为的埃及伊蚊很少飞行超过50米且控制行动可安全基于此假设形成对比。在一栋21层公寓楼的12层进行释放，每层都设有诱蚊产卵器，结果显示蚊虫同样能轻松快速地扩散至楼顶和楼底。
